Active GMP Recombinant Human VSIR protein

• Cat.No.: VSIR-303HG
• Product Overview: GMP Recombinant Human VSIR protein(NP_071436.1)(116 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: HEK293
• Protein Length: DNA sequence encoding B7-H5(GenBank: AAH20568.1 ) was expressed with His tag at the C-terminal.
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: The activity of ED50 is determined by measuring its ability to inhibit the secretion of IL-2 induced by anti-CD3 monoclonal antibody in human T lymphocytes, and the ED50 of this effect is 1-6ug/mL
• Molecular Mass: Recombinant Human B7-H5 protein contains 116 aa. It has a predicted MW of 19.8 kDa.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VSIR V-set immunoregulatory receptor [ Homo sapiens (human) ]
• Official Symbol: VSIR
• Synonyms: B7-H5; SISP1; Gi24; VISTA
• Gene ID: 64115
• mRNA Refseq: NM_022153.2
• Protein Refseq: NP_071436.1
• MIM: 615608
• UniProt ID: Q9H7M9