Active GMP Recombinant Human ERBB2 protein

• Cat.No.: ERBB2-111HG
• Product Overview: GMP Recombinant Human ERBB2 protein(NP_001005862)(630 aa), fused with His Tag at the C-terminal, was expressed in HEK293 cell in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: HEK293
• Protein Length: DNA sequence encoding Human HER2(UniProtKB - P04626) was expressed with His tag at the C-terminal.
• Form: Freeze-dried powder contains Sterile PBS (pH 7.4) with 6% mannitol.
• Bio-activity: The concentration of immobilized human Her2 (His tag) is 100 ng/mL (100uL/well), and the linear binding range that can bind to Her2 antibody is 0.1-5 ng/mL.
• Molecular Mass: Recombinant Human HER2 protein contains 630aa. It has a predicted MW of 71kDa.
• Endotoxin: <0.1EU/ug
• Purity: >=95% by SDS-PAGE and HPLC
• Storage: The lyophilized preparation can be stored at 4°C for 24 months, and the dissolved liquid can be stored at -20°C for 6-12 months to avoid repeated freezing and thawing
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ERBB2 v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) [ Homo sapiens ]
• Official Symbol: ERBB2
• Synonyms: ERBB2; CD340; HER-2/neu; MLN19; NEU; NGL; TKR1
• Gene ID: 2064
• mRNA Refseq: NM_001005862
• Protein Refseq: NP_001005862
• MIM: 164870
• UniProt ID: P04626

Q&As

Do you have a recommended protocol for immobilizing any avi-tagged recombinant proteins on a streptavidin coated plate?

1.Prepare the streptavidin-coated plate by washing it with PBS (phosphate-buffered saline) or another suitable buffer to remove any impurities. 2.Dilute the Avi-tagged protein in the desired buffer at the desired concentration. It is important to use a buffer that is compatible with the protein and does not interfere with its activity or stability. 3.Add the diluted protein to the streptavidin-coated plate and incubate it for a suitable amount of time at room temperature or 4°C. The exact conditions will depend on the specific protein and the desired level of immobilization. 4.Wash the plate with a suitable buffer to remove any unbound protein. 5.Block the remaining binding sites on the plate with a suitable blocking agent such as BSA (bovine serum albumin) or casein. 6.Wash the plate again to remove any unbound blocking agent. 7.Use the immobilized protein for further experiments such as ELISA, Western blotting, or other assays.