Active GMP Recombinant Human DKK1 Protein

• Cat.No.: DKK1-01HG
• Product Overview: Recombinant human DKK1 protein, was expressed in CHO-K1 cell-derived in an animal component free process under cGMP guidelines.

Specification

• Species: Human
• Source: CHO
• Description: Dickkopf-associated protein 1 (Dkk-1) is the initial member of the Dickkopf protein family, which includes Dkk-1, Dkk-2, Dkk-3, Dkk-4 and the related protein Soggy. dkk protein is a secreted protein with two conserved cysteine-rich structural domains. Each structural domain contains 10 cysteine residues. Human mature Dkk-1 is a glycosylated protein with a molecular weight of 40 KD. Dkk-1 acts as an antagonist of Wnt signaling, and Dkk-1 is expressed throughout development and antagonizes Wnt-7a during limb development. The balance between Wnt signaling and Dkk-1 inhibition is critical for bone formation and internal environmental stability. Insufficient or excessive Dkk-1 activity in bone leads to increased or decreased BMD, respectively. In adults, Dkk-1 is expressed in osteoblasts, osteocytes and neurons. Brain ischemia induces Dkk-1 expression, leading to neuronal death.
• Form: Lyophilized powder
• Endotoxin: <20 EU/mg
• Bio-activity: >5.0 x 10^4 IU/mg
• Purity: >95.0% by high performance liquid chromatography (SEC-HPLC) and SDS-PAGE.
• Storage Buffer: 10mM Phosphate-Buffered Saline (pH 7.2)
• Storage: Stable storage at 4℃ for 7-10 days; for long-term storage, please store at -20℃ after dispensing.
• Sterility: Sterility
• Instructions: It is recommended that lyophilized GMP rhDKK1 be dissolved in water for injection or sterilized ultrapure water at a concentration of not less than 100 μg/ml, pending further dilution to working concentration.

Gene Information

• Gene Name: DKK1 dickkopf WNT signaling pathway inhibitor 1 [ Homo sapiens (human) ]
• Official Symbol: DKK1
• Synonyms: SK; DKK-1
• Gene ID: 22943
• mRNA Refseq: NM_012242.4
• Protein Refseq: NP_036374.1
• UniProt ID: O94907
• MIM: 605189