Acitve Recombinant Human NGF protein(Met1-Ala241)

• Cat.No.: NGF-1259H
• Product Overview: Recombinant Human NGF (NP_002497.2, with mutation Arg 121 Gly) (Met1-Ala241) was expressed in HEK293.

Specification

• Source: HEK293
• Species: Human
• Tag: Non
• Protein length: Met1-Ala241
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell prolifeRation assay using TF-1 Human erythroleukemic cells. The ED50 for this effect is typically 5-20 ng/mL.
• Molecular Mass: The recombinant Human NGF consists of 223 amino acids and predicts a molecular mass of 24.8 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
• Official Symbol: NGF
• Synonyms: NGF; nerve growth factor (beta polypeptide); NGFB; beta-nerve growth factor; nerve growth factor, beta subunit; HSAN5; Beta-NGF; MGC161426; MGC161428
• Gene ID: 4803
• mRNA Refseq: NM_002506
• Protein Refseq: NP_002497
• MIM: 162030
• UniProt ID: P01138