Acid Phosphatase Activity Colorimetric Assay Kit

• Cat.No.: Kit-2280
• Product Overview: Acid phosphatases (AP) dephosphorylate phosphate groups from phosphate esters under acid conditions. Different acid phosphatase isozymes are found in different organs, and their serum levels are used as a diagnostic for disease in the corresponding organs. Elevated prostatic acid phosphatase levels may indicate the presence of prostate cancer and elevated tartrate-resistant acid phosphatase levels may indicate the bone disease. Acid Phosphatase Assay Kit is a high sensitivity, simple, direct and HTS-ready colorimetric assay designed to measure AP activity in serum and other samples. It is suitable for research and drug discovery. The kit uses p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax = 405 nm) when dephosphorylated by AP. The kit can detect μU acid phosphatase activity in samples.

Specification

• Tag: Non
• Applications: Phosphate concentrations between 1 μM and 1 mM, with a lower limit of detection of approximately 0.1 nmol, can be directly determined.
• Storage: -20°C
• Shipping: Gel Pack
• Size: 500 assays
• Kit Components: AP Assay Buffer; pNPP Substrate (10 TAB); AP Enzyme; Stop Solution
• Target Species: Mammalian
• Detection method: Absorbance (405 nm)
• Features & Benefits: Simple procedure; takes ~ 3 hours;
  Fast and convenient;
  The assay is sensitive and stable;
  The TUNEL-based assay kit provides complete components including positive and negative control cells for convenient detection of DNA fragmentation in cultured cells and tissue sections.