Acetyl Coenzyme A Assay Kit, colorimetric

• Cat.No.: KITZ-065
• Product Overview: Acetyl Coenzyme A Assay Kit is a quantitative assay kit for measuring Acetyl-CoA in various biological samples, especially animal or plant tissues or cell cultures (adherent or suspension). The detection principle is that malate dehydrogenase can catalyze the reaction of malate acid and NAD+ to produce oxaloacetic acid and NADH. Citrate synthase catalyzes the reaction of acetyl-CoA and oxaloacetic acid to produce citric acid and CoA. Based on the coupling reaction of malate dehydrogenase and citrate synthase, acetyl-CoA content is proportional to the generation of NADH. NADH has an absorption peak at 340 nm, and the measurement of the absorbance value of 340 nm enables calculation of the content of acetyl-CoA.

Specification

• Size: 96-well
• Storage: Shipped at 4°C. Store at -20°C in the dark.
• Shipping: Ice pack
• Kit Components: Extraction Buffer
  Reagent I
  Reagent II
  Reagent III
  Reagent IV
  Standard