2-Deoxyglucose Colorimetric Assay Kit

• Cat.No.: Kit-0003
• Product Overview: 2-Deoxyglucose Assay Kit (Colorimetric) is a quantitative colormetric determination of 2-deoxyglucose at 412 nm.

Specification

• Tag: Non
• Description: Glucose uptake is an important biological tool for studying cell signaling and glucose metabolism. Of many different methods available for measuring glucose uptake, 2-deoxyglucose (2-DG) has been most widely used because of its structural similarity to glucose. As with glucose, 2-DG can be taken up by glucose transporters and metabolized to 2-DG-6-phosphate (2-DG6P). 2-DG6P, however, cannot be further metabolized, and thus accumulates in the cells. The accumulated 2-DG6P is directly proportional to 2-DG (or glucose) uptake by cells.In 2-Deoxyglucose Assay Kit (Colorimetric), 2-DG6P is oxidized to generate NADPH, which is determined by an enzymatic recycling amplification reaction. This easy to use non-radioactive kit is highly sensitive and can detect glucose uptake as low as 10 pmol/sample.
• Applications: Measurement of glucose uptake in response to insulin, growth factors, cytokines, mitogens, and nutrients, etc. Analysis of glucose metabolism and cell signaling in various cell types. Screening anti-diabetic drugs.
• Target Species: Mammals
• Usage: For research use only (RUO)
• Storage: Store kit at -20°C, protected from light. Warm all Buffers to room temperature before use. Briefly centrifuge all small vials prior to opening.
• Kit Components: Extraction Buffer. Cap code: NM. 17 mlNeutralization Buffer. Cap code: clear. 2.5 ml2-Deoxyglucose (2-DG, 10 mM). Cap code: purple. 1 mlAssay Buffer. Cap code: WM. 10 mlEnzyme Mix (Lyophilized). Cap code: orange. 1 vialRecycling Mix (Lyophilized). Cap code: blue. 1 vial2-DG6P Standard (Lyophilized). Cap code: yellow. 1 vialGlutathione Reductase (Lyophilized). Cap code: green. 2 vialsSubstrate-DTNB (Lyophilized). Cap code: red. 2 vials
• Detection method: Colorimetric
• Compatible Sample Types: Attached Cell, Suspension Cell