TRIB2
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Official Full Name
tribbles homolog 2 -
Overview
This gene encodes one of three members of the Tribbles family. The Tribbles members share a Trb domain, which is homologous to protein serine-threonine kinases, but lacks the active site lysine and probably lacks a catalytic function. The Tribbles proteins interact and modulate the activity of signal transduction pathways in a number of physiological and pathological processes. This Tribbles member induces apoptosis of cells mainly of the hematopoietic origin. It has been identified as a protein up-regulated by inflammatory stimuli in myeloid (THP-1) cells, and also as an oncogene that inactivates the transcription factor C/EBPalpha (CCAAT/enhancer-binding protein alpha) and causes acute myelogenous leukemia. Alternatively spliced transcript variants have been found for this gene. -
Synonyms
TRIB2;tribbles homolog 2 (Drosophila);tribbles homolog 2;GS3955;TRB2;C5FW;FLJ57420
Recombinant Proteins
- Human
- Mouse
- Chicken
- Insect Cells
- E.coli
- Mammalian Cells
- Sf9 Cells
- Human
- HEK293
- Human Cells
- GST&His
- GST
- His
- Non
- Flag
- Avi&Fc&His
- DDK&Myc
Background
What is TRIB2 Protein?
TRIB2, known as Tribble Homolog 2, belongs to the Tribble protein family and is considered a pseudokinase, meaning it doesn't have the usual enzyme activity. Still, it's key in cell signaling through interactions with other molecules. TRIB2 helps manage how cells grow, differentiate, and die, and it's connected to different diseases. In cancer, imbalanced TRIB2 levels can encourage tumors to grow and spread. Its expression might also influence the progression of some neurodegenerative disorders. Besides this, TRIB2 plays a role in immune system regulation and metabolism, making it a potential target for treatment strategies.
Fig1. Structure and function of TRIB2 pseudokinase. (Victor Mayoral-Varo, 2021)
What is the Function of TRIB2 Protein?
TRIB2, known as Tribble Homolog 2, belongs to the Tribble protein family and is considered a pseudokinase, meaning it doesn't have the usual enzyme activity. Still, it's key in cell signaling through interactions with other molecules. TRIB2 helps manage how cells grow, differentiate, and die, and it's connected to different diseases. In cancer, imbalanced TRIB2 levels can encourage tumors to grow and spread. Its expression might also influence the progression of some neurodegenerative disorders. Besides this, TRIB2 plays a role in immune system regulation and metabolism, making it a potential target for treatment strategies.TRIB2 Related Signaling Pathway
TRIB2 protein is connected to several crucial cell signaling pathways. It interacts with the PI3K/Akt pathway, affecting cell growth and survival by putting a brake on this pathway. TRIB2 also helps regulate the MAPK/ERK pathway, key for how cells multiply and differentiate. Through these interactions, TRIB2 can influence a range of cell activities, like the cell cycle and cell death. Its role in these signaling pathways makes TRIB2 significant in normal cell function and in disease situations. In cancer, abnormal levels of TRIB2 might boost tumor growth and spread by altering these pathways.TRIB2 Related Diseases
TRIB2 protein is closely linked to various diseases, particularly cancer. It's notably involved in cancers like leukemia, prostate cancer, and liver cancer, where its overexpression is tied to how tumors grow, invade, and spread. TRIB2 helps tumor cells multiply and survive by affecting pathways such as PI3K/Akt and MAPK/ERK, and it reduces cell death. Additionally, in neurodegenerative diseases, abnormal TRIB2 levels might cause neuron problems and damage. In heart-related diseases, TRIB2 could play a part in atherosclerosis, influencing blood vessel health. Thus, TRIB2 serves as a key disease marker and offers a potential target for treatment.Bioapplications of TRIB2
TRIB2 protein holds a lot of promise in biomedicine. It's being looked at as a target for cancer treatment, especially since its high levels are linked to tumor growth, spread, and resistance to treatments in cancers like melanoma, leukemia, and glioblastoma. Researchers are working on selective degraders targeting TRIB2, such as PROTACs, which effectively halt cell growth and trigger cell death. TRIB2 is also being explored for treating liver cancer in diabetes patients by influencing the HBP pathway to control disease progression. These studies open up new ideas and strategies for using TRIB2 in disease treatment.Case Study
Case Study 1: Hou Z. et al. Mol Cancer. 2018
TRIB2 plays a role in colorectal cancer (CRC) by affecting cell growth and drug resistance, but its link to cellular aging in CRC remains unclear. Research shows TRIB2 levels are higher in CRC tissues, correlating with worse outcomes. Lowering TRIB2 levels can slow cancer growth, arrest the cell cycle, and boost cell aging, while more TRIB2 has the opposite effect. It'll link with AP4, suppressing p21 to push cell growth. Cutting AP4 counters TRIB2's effect on cell aging.-
Fig1. Western blot of TRIB2 in SW48 and LoVo cells transfected with TRIB2-specific siRNA. -
Fig2. In vitro GST pull down assay to verify the binding of TRIB2 and AP4.
Case Study 2: Foscarini A. et al. Genes (Basel). 2023
Tribbles pseudokinases (TRIB1-3) are key players in several cancers, but their role in gastric cancer (GC) wasn't clear. Research using TCGA data found that CIN tumors show lower TRIB2 but higher TRIB3 levels compared to MSI-high tumors, with TRIB1 being similar in both. In CIN tumors, low TRIB2 links to more aggressive cancer stages. With no existing TRIB2 studies in GC, the focus here was on its role, finding that overexpressing TRIB2 reduced growth and mobility in certain cancer cell lines, indicating a potential tumor-suppressing function in this context.-
Fig3. WB showing endogenous and ectopic (fused with GFP) TRIB2 protein expression in untransfected parental. -
Fig4. TRIB2 OE effect on cell migration.
Quality Guarantee
High Purity
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Fig1. SDS-PAGE (TRIB2-3646H) -
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Fig2. SDS-PAGE (TRIB2-1626HFL)
Involved Pathway
TRIB2 involved in several pathways and played different roles in them. We selected most pathways TRIB2 participated on our site, such as , which may be useful for your reference. Also, other proteins which involved in the same pathway with TRIB2 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|
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Fig1. The role of TRIB2 in the physiological proliferation of granulosa cells, myoblasts, naive T cells, and thymocytes, and pathological proliferation of VSMCs. (Wenkang Zhang, 2025) -
Fig2. Structural mechanism of Trib2. (Yu Fang, 2021)
Protein Function
TRIB2 has several biochemical functions, for example, mitogen-activated protein kinase kinase binding,NOT nucleotide binding,NOT protein kinase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by TRIB2 itself. We selected most functions TRIB2 had, and list some proteins which have the same functions with TRIB2. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| transcription factor binding | SMARCD3,GATA3,MYBBP1A,MYOD1,RBL1,APBB2,UBN1,RBPJ,KLF5,HYAL1 |
| protein kinase inhibitor activity | CAMK2N1A,CD36,TAOK3,CHP,SH3BP5L,WNK1,TESC,CISHB,SOCS3B,SH3BP5 |
| NOT nucleotide binding | PXK |
| mitogen-activated protein kinase kinase binding | DLG1,MAPK8IP3,RAF1,MAP3K11,ARRB1,IGBP1,TAOK2,TRIB3,BRAF,PIN1 |
| ubiquitin protein ligase binding | ATXN3,UBE2L3,GABARAPL1,XBP1,MUL1,NEDD8,UBE2V2,RANGAP1,POLR2A,UBE2K |
| ubiquitin-protein transferase regulator activity | TRIB3,TRIB1 |
| NOT protein kinase activity | TRIB1,STRADB,PXK,STRADA,HSPB8,MLKL |
Interacting Protein
TRIB2 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with TRIB2 here. Most of them are supplied by our site. Hope this information will be useful for your research of TRIB2.
LRRK2;BTRC;PRMT6;SLAMF7;UBQLN4;CARD16;TCF4;CTNNB1;RFWD2;SMURF1
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References
- Lind, A; Ramelius, A; et al. A/H1N1 antibodies and TRIB2 autoantibodies in narcolepsy patients diagnosed in conjunction with the Pandemrix vaccination campaign in Sweden 2009-2010. JOURNAL OF AUTOIMMUNITY 50:99-106(2014).
- Katzav, A; Arango, MT; et al. Passive transfer of narcolepsy: Anti-TRIB2 autoantibody positive patient IgG causes hypothalamic orexin neuron loss and sleep attacks in mice. JOURNAL OF AUTOIMMUNITY 45:24-30(2013).
