PRKCH
-
Official Full Name
protein kinase C, eta -
Overview
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipids-dependent protein kinase. It is predominantly expressed in epithelial tissues and has been shown to reside specifically in the cell nucleus. This protein kinase can regulate keratinocyte differentiation by activating the MAP kinase MAPK13 (p38delta)-activated protein kinase cascade that targets CCAAT/enhancer-binding protein alpha (CEBPA). It is also found to mediate the transcription activation of the transglutaminase 1 (TGM1) gene. -
Synonyms
PRKCH;protein kinase C, eta;protein kinase C eta type;PKC-L;nPKC-eta;protein kinase C-eta;MGC93383
Recombinant Proteins
- Human
- Mouse
- Rat
- E.coli
- Insect Cell
- Sf9 Insect Cell
- Mammalian Cell
- HEK293
- Insect (sf21)
- His
- His&GST
- Non
- GST
- Flag
- His&Strep
- His&Fc&Avi
Background
What is PRKCH protein?
PRKCH belongs to the η subtype of protein kinase C (PKC) family. PKC family is an important class of cellular signal transduction kinases. PRKCH participates in multiple cellular signaling pathways such as Wnt pathway and tumor necrosis factor signaling pathway, regulating biological processes like cell proliferation, differentiation and apoptosis. In the liver, PRKCH expression level is high and can promote fatty acid synthesis and lipid droplet formation in fatty cells. Loss of PRKCH can reduce the degree of fatty liver.
What is the function of PRKCH protein?
Cell signaling: PRKCH acts as a serine/threonine kinase and participates in important cellular signaling pathways like Wnt and TNF signaling. This regulates processes like proliferation, differentiation, and apoptosis.
Lipid metabolism: PRKCH promotes fatty acid synthesis and lipid droplet formation in liver and fat cells. It plays a role in triglyceride and phospholipid biosynthesis.
Fatty liver disease: Higher expression of PRKCH in the liver contributes to fatty acid accumulation and hepatic steatosis (fatty liver). Loss of PRKCH reduces fatty liver severity.
Inflammation: PRKCH activation upregulates pro-inflammatory cytokines like TNFα. This enhances inflammation in conditions like non-alcoholic fatty liver disease (NAFLD).
Atherosclerosis: It influences atherogenesis by affecting foam cell formation, inflammatory pathways in endothelial cells and vascular smooth muscle cells.
PRKCH related signaling pathway
Wnt/β-catenin pathway: PRKCH phosphorylates and activates Dishevelled, leading to β-catenin stabilization and transcriptional regulation of Wnt target genes.
TNF signaling pathway: PRKCH enhances TNFα-induced NF-κB activation by phosphorylating IκB kinase. This promotes inflammation.
PI3K/AKT pathway: PRKCH activation can positively regulate the PI3K/Akt survival pathway in some contexts.
MAPK pathways: PRKCH modulates the ERK, JNK and p38 MAPK pathways involved in proliferation, apoptosis and inflammatory responses.
PRKCH Related Diseases
Non-alcoholic fatty liver disease (NAFLD): Higher PRKCH expression drives hepatic steatosis in NAFLD. Its inhibition helps resolve fatty liver.
Atherosclerosis: PRKCH promotes foam cell formation, inflammation and plaque development in atherosclerotic lesions.
Obesity: PRKCH expression is elevated in obese adipose tissue and liver. It links obesity to metabolic syndrome pathogenesis.
Insulin resistance/diabetes: PRKCH disruption enhances insulin sensitivity and glucose tolerance, suggesting a role in diabetes.
Biomedical Application of PRKCH Protein
- Disease biomarker: PRKCH expression levels could serve as an indicator of diseases like NAFLD, atherosclerosis and cancer progression.
- Drug target: Developing selective small molecule PRKCH inhibitors may provide therapeutic benefits for metabolic diseases, inflammation and cancer.
- Gene therapy: Correcting defective/overexpressed PRKCH genes may help treat related genetic disorders.
- Cell-based therapies: Delivering PRKCH-modulated cells could aid conditions involving PRKCH dysregulation like fatty liver.
Case Study
(Fangning Pang, 2022)
Fig3. Expression of PRKCH mRNA in glioma and paired normal samples from GEPIA.
(Kumiko Torisu, 2016)
Fig4. Quantification of Sudan IV-positive lipid droplets in the liver. Five mice of each genotype were examined.
Quality Guarantee
High Purity
Fig1. SDS-PAGE (Cat. No.: PRKCH-1440HF)
High Bioactivity & Detection Sensitivity
Fig2. The activity was measured by off-chip mobility shift assay. The enzyme was incubated with fluorecence-labeled substrate, Mg(or Mn)/ATP, and Lipid Activator. The phosphorylated and unphosphorylated substrates were separated and detected by LabChip™3000.
Involved Pathway
PRKCH involved in several pathways and played different roles in them. We selected most pathways PRKCH participated on our site, such as Vascular smooth muscle contraction,Tight junction,Inflammatory mediator regulation of TRP channels, which may be useful for your reference. Also, other proteins which involved in the same pathway with PRKCH were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Inflammatory mediator regulation of TRP channels | ASIC2,PIK3R5,IL1B,TRPA1,CYP2C38,PRKCA,P2RY2,CYP2C55,ACCN5,MAPK8 |
| Tight junction | CLDNF,MRAS,CSNK2A2A,PARD6A,PPP2CB,MYH7B,CLDN6,PRKCG,PRKCI,RHOAD |
| Vascular smooth muscle contraction | MYL12.1,CACNA1SA,CALD1B,PLCB3,PLCB2,PPP1CAB,CALM3,PPP1CC,GNA12,PPP1R14AB |
Protein Function
PRKCH has several biochemical functions, for example, ATP binding,Ral GTPase binding,calcium-independent protein kinase C activity. Some of the functions are cooperated with other proteins, some of the functions could acted by PRKCH itself. We selected most functions PRKCH had, and list some proteins which have the same functions with PRKCH. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| ATP binding | EIF4A1B,PMVK,NLRX1,ATP2A1,PDK3,ACSM3,RET,YME1L1,ATP13A5,ABCD3B |
| enzyme binding | HDAC6,ANK1,PARP4,SCNM1-PS,UGT1A6A,U2AF2,HLCS,PCCA,PPP1R3D,BCR |
| protein kinase C activity | PRKCHA,PRKD1,PRKCBB,PRKCZ,PKN2,PRKCB,PRKD2,PRKD3,PRKCQ,PRKCBA |
| calcium-independent protein kinase C activity | PRKCE,PRKCD |
| Ral GTPase binding | EXOC4,RAB34,RNF41,EXOC8,MYO1C,PIH1D2,USP33,EXOC5,RALBP1,LSM2 |
| metal ion binding | PLCH2,ITGA2,ZNF577,GLRX5,ZFP827,EGR2B,CAR7,PM20D1.2,ZNF821,G2E3 |
Interacting Protein
PRKCH has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PRKCH here. Most of them are supplied by our site. Hope this information will be useful for your research of PRKCH.
ndb25_parsc;HSP90AB1;glpD;ZBTB8OS
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