FBP1

What is FBP1 protein?

FBP1 (fructose-bisphosphatase 1) gene is a protein coding gene which situated on the long arm of chromosome 9 at locus 9q22. Fructose-1,6-bisphosphatase 1(FBP1) is a gluconeogenesis regulatory enzyme, catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate and inorganic phosphate. The FBP1 protein is consisted of 338 amino acids and its molecular mass is approximately 36.8 kDa.

What is the function of FBP1 protein?

FBP1 appears to modulate glycerol gluconeogenesis in liver. It is an important regulator of appetite and adiposity. The expression of FBP1 in liver will increase after nutrient excess increases circulating satiety hormones and reduces appetite-stimulating neuropeptides and thus it seems to provide a feedback mechanism to limit weight gain.

Fig1. A schematic diagram illustrates how reduced FBP1 expression upon irradiation leads to glycolysis activation and infiltration of glioblastoma (GBM). (Beomseok Son, 2019)

FBP1 Related Signaling Pathway

The signaling pathways involved in FBP1 (fructose-1, 6-diphosphatase) are gluconeogenesis and glycolysis. In addition, the activity of FBP1 is regulated by many factors, including AMP, Insulin, PPARα, etc. PPARα is a nuclear receptor whose activation can inhibit FBP1 transcription. AMPK is an energy-sensitive kinase whose activity is regulated by changes in the intracellular AMP/ATP ratio. Activated AMPK can inhibit the expression and activity of FBP1, thereby reducing gluconeogenesis.

FBP1 Related Diseases

FBP1 is mainly involved in the metabolic process of glucose. If the function of this enzyme is abnormal, or the expression of FBP1 is low, it is associated with hypoglycemia and metabolic acidosis. And it may affect the normal metabolism of glucose, which may lead to a series of metabolic diseases, such as diabetes and obesity. Other related diseases include glycogen storage disease type I (GSD-I), hepatic insulin resistance and hepatocellular carcinoma (HCC).

Bioapplications of FBP1

FBP1 is widely used in biology, involving many fields such as glucose metabolism, diabetes, drug research and development. By further studying the function and regulatory mechanism of FBP1, we can provide new ideas and methods for the prevention, diagnosis and treatment of related diseases.

Case Study 1: Alexander Damanakis, 2022

Fig1. Representative images of immunohistochemistry (IHC) from A FBP1-negative (score 0) and B FBP1-positive (score 3) specimens of esophageal adenocarcinoma from the tissue microarray.

Case Study 2: Bo Wang, 2020

Fig2. MCF-7, T47D, VCaP and PC-3 cells were harvested for western blot after infected with lentivirus expressing control shRNA or FBP1-specific shRNA.

FBP1 has several biochemical functions, for example, AMP binding,fructose 1,6-bisphosphate 1-phosphatase activity,identical protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by FBP1 itself. We selected most functions FBP1 had, and list some proteins which have the same functions with FBP1. You can find most of the proteins on our site.

FBP1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with FBP1 here. Most of them are supplied by our site. Hope this information will be useful for your research of FBP1.

BIN1;FBP2;ACTN3;DYNC1I1;BUB1;TLR2