DUT

  • Official Full Name

    deoxyuridine triphosphatase

  • Overview

    This gene encodes an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP. Elevated levels of dUTP lead to increased incorporation of uracil into DNA, which induces extensive excision repair mediated by uracil glycosylase. This repair process, resulting in the removal and reincorporation of dUTP, is self-defeating and leads to DNA fragmentation and cell death. Alternative splicing of this gene leads to different isoforms that localize to either the mitochondrion or nucleus. A related pseudogene is located on chromosome 19. This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA.

  • Synonyms

    DUT;deoxyuridine triphosphatase;dUTP pyrophosphatase;deoxyuridine 5-triphosphate nucleotidohydrolase, mitochondrial;dUTPase;dUTP nucleotidohydrolase;FLJ20622;DYNLL1;dynein, light chain, LC8-type 1;LC8;PIN;DLC1;DLC8;LC8a;DNCL1;hdlc1;DNCLC1;MGC126137;MGC126138;dynein light chain 1, cytoplasmic;8 kDa dynein light chain;cytoplasmic dynein light polypeptide;dynein, cytoplasmic, light polypeptide 1;protein inhibitor of neuronal nitric oxide synthase;Dynein light chain LC8-type 1

Recombinant Proteins

  • Human
  • Streptomyces coelicolor A3(2)
  • Canis lupus
  • Zebrafish
  • Rat
  • Chicken
  • Mouse
  • E.coli
  • E.Coli/Yeast
  • Mammalian Cells
  • HEK293
  • Wheat Germ
  • In Vitro Cell Free System
  • GST
  • Non
  • His
  • Myc&DDK
Cat.# Product name Source (Host) Species Tag Protein Length Price
DUT-12221H Recombinant Human DUT, GST-tagged E.coli Human GST 89-252a.a.
dUTPase-88 Active Recombinant Thermostable dUTPase E.coli Human Non
DUT-1034S Recombinant Streptomyces coelicolor A3(2) DUT protein, His-tagged E.Coli/Yeast Streptomyces coelicolor A3(2) His
DUT-12C Recombinant Canis lupus DUT protein, His-tagged E.coli Canis lupus His
DUT-1524Z Recombinant Zebrafish DUT Mammalian Cells Zebrafish His
DUT-1979R Recombinant Rat Dut protein, His-tagged HEK293 Rat His
DUT-27878TH Recombinant Human DUT, His-tagged E.coli Human His 183 amino acids
DUT-2950H Recombinant Human DUT Protein, GST-tagged Wheat Germ Human GST
DUT-3527C Recombinant Chicken DUT Mammalian Cells Chicken His
DUT-76H Recombinant Human DUT protein, GST-tagged E.coli Human GST 70-252
DUT-77H Recombinant Human DUT protein, His-tagged E.coli Human His 70-252
DUT-78H Recombinant Human DUT protein E.coli Human Non 70-252
DUT-790H Recombinant Human DUT protein E.coli Human Non Met1-Asn164
DUT-791H Recombinant Human DUT protein, His-tagged E.coli Human His Met1-Asn164
DUT-792H Recombinant Human Deoxyuridine Triphosphatase, His-tagged E.coli Human His
DUT-6768HCL Recombinant Human DUT 293 Cell Lysate HEK293 Human Non
DUT-3772H Recombinant Human DUT Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293 Human Myc&DDK
DUT-4123HF Recombinant Full Length Human DUT Protein, GST-tagged In Vitro Cell Free System Human GST Full L. 252 amino acids
Dut-449M Recombinant Mouse Dut Protein, MYC/DDK-tagged HEK293 Mouse Myc&DDK
DUT-6598H Recombinant Human DUT protein, His-tagged E.coli Human His 156aa(including fusion tag)

    Involved Pathway

    DUT involved in several pathways and played different roles in them. We selected most pathways DUT participated on our site, such as Metabolic pathways,Metabolism,Metabolism of nucleotides, which may be useful for your reference. Also, other proteins which involved in the same pathway with DUT were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

    Pathway Name Pathway Related Protein
    superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis CMPK2
    pyrimidine deoxyribonucleotides de novo biosynthesis DTYMK
    Pyrimidine metabolism POLE2,NT5C2B,POLD2,CANT1B,PNP5A,TYMP,POLR2EB,UMPS,POLR2GL,ITPA
    Metabolic pathways ACADM,SGMS2,TDO2B,AGPAT3,ETNK2,ACSM4,COX6C,IMPDH2,NADKA,ACOT1
    Pyrimidine biosynthesis DHODH
    Metabolism of nucleotides TYMP,DPYDA.1,ADSS,ADK,DPYS,DHODH,AK7,ADA,ITPA,GLRX
    Metabolism CERS3B,Sult2a6,ADA,AGL,SLC25A2,CYP2K22,NUDT16,CDS1,CYP8B2,AS3MT

    Protein Function

    DUT has several biochemical functions, for example, dUTP diphosphatase activity,magnesium ion binding,poly(A) RNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by DUT itself. We selected most functions DUT had, and list some proteins which have the same functions with DUT. You can find most of the proteins on our site.

    Function Related Protein
    protein binding NPC1,RPS6,PSMB5,TTN,C7orf42,H2-KE2,MIB1,METTL21C,ZKSCAN4,HNRPLL
    magnesium ion binding PDXK,NUDT5,TSSK2,GTPBP5,SERPINB1A,GLUL,PRPS1,NUDT16,ERN2,ENO1
    poly(A) RNA binding TSNAX,ZFP326,ANKHD1,BTF3,EIF5A,NOA1,EDF1,NARG1,SRP54B,STIP1

    Interacting Protein

    DUT has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with DUT here. Most of them are supplied by our site. Hope this information will be useful for your research of DUT.

    NUDT18;PLEKHF2;GH1;PINX1;RHOB;parE;q81k60_bacan;recN;ypdA;q81v76_bacan;yacO;q8cki1_yerpe;clpA3;fadB;cysJ;panE;epmA

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