What is RUVC Protein

      RUVC, or more formally RecU-like resolvase, is a protein that belongs to the resolvase family. Also known by synonyms such as "RecU" and "RusA," this protein is renowned for its role in DNA recombination and repair. Recent research advances have shed light on its unique structural characteristics and its classification within the broader protein family.

      RUVC Protein Structural Characteristics and Classification

      The RUVC protein boasts a distinctive structural makeup, characterized by its catalytic domain and DNA-binding motifs. Its catalytic core, essential for its role in recombination, exhibits conserved features across various organisms. Within the resolvase family, RUVC stands out as a key player, showcasing evolutionary adaptations that enable its specific functions.

      Cutting-edge research has expanded our understanding of RUVC's molecular intricacies. Recent studies have unraveled the dynamic interactions between RUVC and DNA, providing insights into the protein's catalytic mechanism. These findings have paved the way for further exploration of RUVC's role in genetic recombination and repair pathways, fostering a deeper appreciation for its significance in cellular processes.

      Model indicating the interactions between the RuvA, RuvB and RuvC proteins and the Holliday junction

      Figure 1. Model indicating the interactions between the RuvA, RuvB and RuvC proteins and the Holliday junction. (van Gool A J, et al., 1998)

      RUVC Biological Functions and Molecular Mechanisms

      The biological functions of RUVC extend beyond its role in DNA recombination. This versatile protein plays a crucial part in resolving Holliday junctions, the pivotal intermediates in genetic recombination. Through its catalytic activity, RUVC facilitates the resolution of DNA structures, ensuring the faithful repair of damaged genetic material.

      RUVC achieves its functions through intricate molecular mechanisms. It recognizes and binds to specific DNA structures, initiating a cascade of events that lead to the resolution of Holliday junctions. This process is finely regulated, highlighting the precision with which RUVC operates in the cellular context.

      RUVC Related Signaling Pathway

      RUVC's participation in signal pathways is intricately connected to its functions in DNA recombination and repair. It collaborates with other proteins and signaling molecules to orchestrate the cellular response to DNA damage. Elucidating the details of RUVC's involvement in these pathways is essential for unraveling the broader network of molecular events that safeguard genomic integrity.

      RUVC Related Diseases

      While RUVC itself is not directly implicated in diseases, dysregulation of DNA recombination and repair pathways in which RUVC participates can contribute to various disorders. Aberrations in these processes may lead to genomic instability, increasing the risk of cancer and other genetic diseases. Understanding the nuances of RUVC's involvement in these pathways is crucial for deciphering the molecular basis of such conditions.

      RUVC's Applications in Biomedicine

      The significance of RUVC extends beyond its roles in fundamental biological processes. In the realm of biomedicine, RUVC has found applications in diagnostics development, vaccine development, and therapeutics.

      • Diagnostics Development

      RUVC's ability to recognize and interact with specific DNA structures makes it a valuable tool in diagnostics. Researchers have leveraged RUVC's binding specificity to design DNA probes for detecting genetic mutations associated with diseases. This application holds promise for the early and accurate diagnosis of genetic disorders.

      • Vaccine Development

      Understanding the molecular intricacies of RUVC's interactions with DNA has implications for vaccine development. Researchers are exploring the use of RUVC as a potential component in DNA-based vaccines, capitalizing on its ability to stimulate specific immune responses. This innovative approach could pave the way for the development of more effective and targeted vaccines.

      • Therapeutics

      The therapeutic potential of RUVC is being explored in the context of genetic diseases and cancer. Targeting the DNA recombination and repair pathways in which RUVC is involved may offer new avenues for developing treatments that address the underlying causes of these conditions. RUVC-based therapeutics could potentially enhance the precision and efficacy of treatment strategies.

      Recommended Products

      Cat.# Product name Species Source (Host) Tag
      RUVC-2517A Recombinant Akkermansia Muciniphila RUVC Protein (1-167 aa), His-Myc-tagged Akkermansia Muciniphila E.coli His/Myc
      ruvC-133E Recombinant E. coli RuvC E.coli E.coli N/A
      RuvC-2666E Recombinant E. coli Holliday Junction DNA Helicase RuvC E.coli E.coli N/A
      ruvC-4233E Recombinant Escherichia coli ruvC protein, His-SUMO-tagged E.coli E.coli His-SUMO
      RUVC-1336S Recombinant Streptomyces coelicolor A3(2) RUVC protein, His-tagged Streptomyces coelicolor A3(2) E. coli or Yeast His

      Reference

      • van Gool A J , et al. Functional interactions between the Holliday junction resolvase and the branch migration motor of Escherichia coli. The EMBO Journal. 1998, 17(6): 1838-1845.