Recombinant HRV3C Protease, GST-tagged

• Cat.No.: HRV3C-366H
• Product Overview: Recombinant Human HRV3C Protease is produced by Insect cells expression system. This protein is fused with a GST tag.

Specification

• Description: Human rhinovirus 3C protease (HRV 3C Protease) is a cysteine protease that recognizes the Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro sequence (also called the PreScission site), and cleaves between Gln and Gly. The recombinant GST-HRV 3C can be used to cleave the GST tag in fusion proteins expressed using the pGEX-6p vector or other vectors, then removed by glutathione resin. It exhibits high specificity and activity at 4 0C in an overnight incubation reaction containing 1 mass unit of HRR 3C and 100 mass units of targeting protein.
• Source: Bacteria
• Species: HRV type 14
• Tag: GST
• Form: 20 mM Tris, 150 mM NaCl, 2 mM βME, 10% Glycerol
• Purity: > 90 % by SDS-PAGE
• Notes: We recommend using 1% supplied HRV 3C protease (w/w) to preform reactions at 4 centigrade for 12-16 h. Depending on specific substrates, HRV 3C protease concentration, incubation time and reaction temperature should be optimized. GST-HRV 3C protease can be removed by glutathione resin after incubation.
• Storage: Store at -80 centigrade; avoid multiple freeze-thaw cycles.