SFPQ
-
Official Full Name
splicing factor proline/glutamine-rich -
Overview
DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as an heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is probably mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO/SF-1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. -
Synonyms
SFPQ;splicing factor proline/glutamine-rich;PSF;POMP100;splicing factor, proline- and glutamine-rich;hPOMp100;100 kDa DNA-pairing protein;PTB-associated splicing factor;PTB-associated-splicing factor;DNA-binding p52/p100 complex, 100 kDa subunit;polypyrimidine tract binding protein associated;polypyrimidine tract-binding protein-associated splicing factor;polypyrimidine tract-binding protein-associated-splicing factor;splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated);splicing factor proline/glutamine rich (polypyrimidine tract-binding protein-associated)
Recombinant Proteins
- Human
- Zebrafish
- Mouse
- E.coli
- Mammalian Cell
- Wheat Germ
- HEK293
- HEK293T
- Mammalian cells
- His
- GST
- Non
- His&Fc&Avi
- Myc&DDK
- Flag
Involved Pathway
SFPQ involved in several pathways and played different roles in them. We selected most pathways SFPQ participated on our site, such as mRNA processing, which may be useful for your reference. Also, other proteins which involved in the same pathway with SFPQ were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| mRNA processing | PRPF4B,RBM12B1,PRPF8,QK,SUGP2,CLK2,DHX15,ZFP830,FAM120B,RBM12B2 |
Protein Function
SFPQ has several biochemical functions, for example, RNA polymerase II distal enhancer sequence-specific DNA binding,chromatin binding,core promoter binding. Some of the functions are cooperated with other proteins, some of the functions could acted by SFPQ itself. We selected most functions SFPQ had, and list some proteins which have the same functions with SFPQ. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| RNA polymerase II distal enhancer sequence-specific DNA binding | HNRNPC,H3F3A,NONO,GZF1,TAL1,BACH1,TFAP2C,RELA,NFE2L1,MED12 |
| histone deacetylase binding | BRMS1L,HEY2,NACC2,HDAC4,KAT2B,CBX5,RELA,SRF,BCOR,ANKRD1 |
| transcription regulatory region DNA binding | TFE3,CARM1,DHX36,SREBF1,TAF9,TNF,MESP1,KLF11,MTERF3,HOXA13 |
| nucleotide binding | HNRPDL,CSTF2T,DYRK1AA,MYHZ2,SRSF1A,DNM1B,RAVER2,ABCG2A,ERBB4A,DAZL |
| core promoter binding | NPAS2,SPI1,REST,PSPC1,GTF2B,CALCOCO1,AGO1,MYOG,CRY1,E2F7 |
| chromatin binding | CAPNS2,STAG2,NOC2L,UPF1,CUX1,MCM5,MEIOB,TP53,OST4,CREBBP |
| transcription regulatory region sequence-specific DNA binding | ZNF658,SUV39H1,NFE2L1B,GATA3,NR5A1A,MKL1,NKX3-1,BAHD1,CRY2,ATF3 |
| poly(A) RNA binding | SRP54B,CANX,DYNC1H1,PPP1R2P9,RBM4B,PPIG,NOC3L,THUMPD1,RBM34,SNIP1 |
| protein binding | SCAF8,SERPINE1,CCNL1,Txn1,USP28,SLX1B,SMAD4,LAMTOR1,DDIT4L,MAP4K5 |
Interacting Protein
SFPQ has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with SFPQ here. Most of them are supplied by our site. Hope this information will be useful for your research of SFPQ.
PTBP1;PTK6;SNRPA;THRAP3;RNF43
Resources
Related Services
Related Products
References
- Chang, HH; Hu, HH; et al. Proteomic analyses and identification of arginine methylated proteins differentially recognized by autosera from anti-Sm positive SLE patients. JOURNAL OF BIOMEDICAL SCIENCE 20:-(2013).
- Kurokawa, K; Tanahashi, T; et al. Role of miR-19b and its target mRNAs in 5-fluorouracil resistance in colon cancer cells. JOURNAL OF GASTROENTEROLOGY 47:883-895(2012).
