GFP
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Official Full Name
Green Fluorescent Protein -
Overview
Green fluorescence protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants. -
Synonyms
GFP;Green Fluorescent Protein
Recombinant Proteins
- Human
- Renilla reniformis
- Aequorea victoria
- Jellyfish Aequorea Victoria
- Bacterial
- Pan-species
- Bovine
- Jellyfish
- E.coli
- P.pastoris
- S.Cerevisiae
- HEK293
- bovine Spinal Cord
- Yeast
- Mammalian Cells
- His
- Avi
- Non
- Flag
- Flag&GST&HA&His&Myc
- GST
- His&SUMO
Involved Pathway
GFP involved in several pathways and played different roles in them. We selected most pathways GFP participated on our site, such as , which may be useful for your reference. Also, other proteins which involved in the same pathway with GFP were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|
Protein Function
GFP has several biochemical functions, for example, . Some of the functions are cooperated with other proteins, some of the functions could acted by GFP itself. We selected most functions GFP had, and list some proteins which have the same functions with GFP. You can find most of the proteins on our site.
| Function | Related Protein |
|---|
Interacting Protein
GFP has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with GFP here. Most of them are supplied by our site. Hope this information will be useful for your research of GFP.
Resources
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References
- Rafiee, MR; Shafaroudi, AM; et al. Enrichment of A Rare Subpopulation of miR-302-Expressing Glioma Cells by Serum Deprivation. CELL JOURNAL 16:494-505(2015).
- Jiang, JM; Lin, YX; et al. Proteomics approach reveals mechanism underlying susceptibility of loquat fruit to sunburn during color changing period. FOOD CHEMISTRY 176:388-395(2015).
