CDK9
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Official Full Name
cyclin-dependent kinase 9 -
Overview
The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS. [provided by RefSeq, Jul 2008] -
Synonyms
CDK9;cyclin-dependent kinase 9;TAK;C-2k;CTK1;CDC2L4;PITALRE;CDC2-related kinase;cell division protein kinase 9;serine/threonine protein kinase PITALRE;cell division cycle 2-like protein kinase 4;tat-associated kinase complex catalytic subunit
Recombinant Proteins
- Human
- Zebrafish
- Rat
- Chicken
- Rhesus macaque
- Wheat Germ
- Insect cell
- Mammalian Cell
- E.coli
- Insect Cell
- Sf9 Insect Cell
- HEK293
- Insect (sf21)
- In Vitro Cell Free System
- GST
- His&GST
- His
- His&T7
- Non
- Flag
- His&Fc&Avi
Background
What is CDK9 protein?
CDK9 (cyclin dependent kinase 9) gene is a protein coding gene which situated on the long arm of chromosome 9 at locus 9q34. The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. The CDK9 protein is consisted of 372 amino acids and its molecular mass is approximately 42.8 kDa.
What is the function of CDK9 protein?
CDK9 protein was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. Phosphorylation of CDK9 regulates DNA damage repair and apoptosis signaling pathways. CDK9 also interacts with proteins related to apoptosis and cell cycle regulation, and is involved in the regulation of cell proliferation and cell fate determination.
Fig1. Schematic demonstrating the role of CDK9 in the transcription elongation process involved in tumor cell survival. (Hangzhan Ma, 2019)
CDK9 Related Signaling Pathway
CDK9 is mainly involved in the process of transcription extension, and can promote gene expression through the kinase activity of CDK9/P-TEFb complex. In addition, CDK9 is involved in cell cycle regulation. When cells enter the S and G2 phases, CDK9 is activated and can promote the cell to enter mitosis.
CDK9 Related Diseases
Diseases associated with CDK9 include Hypothyroidism, Congenital, Nongoitrous, 6 and Nut Midline Carcinoma. Due to its key role in transcriptional regulation, abnormal activity of CDK9 has been linked to a variety of diseases, including certain types of cancer, cardiovascular disease, and viral infections such as HIV.
Bioapplications of CDK9
Because of its potential role in a variety of diseases, CDK9 has become an attractive target for drug development. For HIV treatment in particular, drugs that target CDK9 may help inhibit viral replication.
Case Study
Case Study 1: Tao Guo, 2022
Fig1. CDK9 expression was up-regulated in papillary thyroid carcinoma (PTC) patients.
Case Study 2: Yingjie Qing, 2021
Fig2. The protein expression of p-RNA Pol II (Ser2), p-RNA Pol II (Ser5), CyclinT1, CDK9 and MCL-1 in KU-812 xenografts. β-actin was used as a loading control.
Quality Guarantee
High Purity
Fig1. SDS-PAGE (CDK9-2705H) (PROTOCOL for western blot)
.
Fig2. Activity Data. (CDK9-27909TH)
Involved Pathway
CDK9 involved in several pathways and played different roles in them. We selected most pathways CDK9 participated on our site, such as Disease,Formation of HIV elongation complex in the absence of HIV Tat,Formation of HIV-1 elongation complex containing HIV-1 Tat, which may be useful for your reference. Also, other proteins which involved in the same pathway with CDK9 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| HIV Life Cycle | NMT2,VPS37A,CHMP4C,PSIP1,SSRP1,RCC1,TAF1L,TCEB3,PSTPIP2,NMT1 |
| Formation of RNA Pol II elongation complex | SSRP1,SUPT16H,CTDP1,CCNT2B,RDBP,SUPT4H1,TH1L,TCEA1,ELL,WHSC2 |
| Disease | CHMP4B,FDX1,PDCD6IP,OPN1MW2,EPM2A,FMOD,VPS37A,TCEA1,CCR5,NMT1 |
| Gene Expression | POLR3G,LRPPRC,MBD3,HNRNPL,GLS2B,ELAVL1,HENMT1,ZIK1,ZNF750,MTA1 |
| HIV Infection | PDCD6IP,PACS1,AP1S1,RCC1,NMT1,CHMP4B,NPM1,NMT2,AP1M2,PSIP1 |
| Generic Transcription Pathway | ZNF189,ZNF383,ZNF597,ZNF222,ESRRG,MED13B,ZNF621,COX16,ZNF136,NR2E3 |
| Formation of HIV elongation complex in the absence of HIV Tat | PSTPIP2,RDBP,TCEA1,ELL,TH1L,TCEB3,SSRP1,COBRA1,CTDP1,SUPT5H |
| Formation of HIV-1 elongation complex containing HIV-1 Tat | TCEA1,RDBP,SUPT5H,TH1L,SUPT4H1,SSRP1,WHSC2,CTDP1,COBRA1,TCEB3 |
Fig2. HIV hijacks CDK9 for transcription of its own genome. (Curtis W Bacon, 2019)
Protein Function
CDK9 has several biochemical functions, for example, ATP binding,DNA binding,RNA polymerase II carboxy-terminal domain kinase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by CDK9 itself. We selected most functions CDK9 had, and list some proteins which have the same functions with CDK9. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| transcription regulatory region DNA binding | SOX6,GABPB2,KLF2A,IKZF2,GATA4,TCF7L2,SALL3B,STAT3,SP1,WNT5A |
| chromatin binding | DNMT1,TFAM,HMGB2,RDBP,PPARGC1A,CBX4,SMC1AL,ARX,HDAC5,MB21D2 |
| ATP binding | VMHCL,PSMC1,PRKD1,AK5L,CDK13,TAOK1,PANK1B,MAP2K2A,CDK15,SEPHS2 |
| DNA binding | RYBPA,ZNF444,HDGFRP2,SMAD6A,ETS1,FOXO6,NFIXB,ASCL1A,ZFP367,POLR2B |
| RNA polymerase II carboxy-terminal domain kinase activity | CCNK,CDK12,CDK1,CDK8,CDK13,CDK7,MAPK1 |
| snRNA binding | HEXIM2,RBPMS,NCBP2,SNRNP35,CCNT1,HEXIM1 |
| protein binding | MUC5B,WIPF1,TMEM14C,UBE2D2,FGF7,TOR1A,SIX1,FASTKD5,NOX5,SLC30A3 |
| protein kinase activity | DCLK1A,TNNI3K,PRKCDA,CAMK1DB,Aatk,NPR1A,RPS6KA3A,LTK,ACVR1,ABL2 |
| transcription factor binding | WWP2,ERCC3,PPARG,TCF12,TNFRSF10A,UBE2I,GPX3,FOXA2,NFKBIA,SIRT1 |
Interacting Protein
CDK9 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CDK9 here. Most of them are supplied by our site. Hope this information will be useful for your research of CDK9.
CCNT1;AFF1
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References
- Holcakova, J; Muller, P; et al. Inhibition of Post-Transcriptional RNA Processing by CDK Inhibitors and Its Implication in Anti-Viral Therapy. PLOS ONE 9:-(2014).
- Mbonye, UR; Gokulrangan, G; et al. Phosphorylation of CDK9 at Ser175 Enhances HIV Transcription and Is a Marker of Activated P-TEFb in CD4(+) T Lymphocytes. PLOS PATHOGENS 9:-(2013).
