CAT
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Official Full Name
Chloramphenicol acetyltransferase -
Overview
Chloramphenicol acetyltransferase (or CAT) is a bacterial enzyme (EC 2.3.1.28) that detoxifies the antibiotic chloramphenicol and is responsible for chloramphenicol resistance in bacteria. This enzyme covalently attaches an acetyl group from acetyl-CoA to chloramphenicol, which prevents chloramphenicol from binding to ribosomes. A histidine residue, located in the C-terminal section of the enzyme, plays a central role in its catalytic mechanism. The crystal structure of the type III enzyme from Escherichia coli with chloramphenicol bound has been determined. CAT is a trimer of identical subunits (monomer Mr 25,000) and the trimeric structure is stabilised by a number of hydrogen bonds, some of which result in the extension of a beta-sheet across the subunit interface. Chloramphenicol binds in a deep pocket located at the boundary between adjacent subunits of the trimer, such that the majority of residues forming the binding pocket belong to one subunit while the catalytically essential histidine belongs to the adjacent subunit. His195 is appropriately positioned to act as a general base catalyst in the reaction, and the required tautomeric stabilisation is provided by an unusual interaction with a main-chain carbonyl oxygen. -
Synonyms
E. coli Chloramphenicol acetyltransferase;E. coli CAT;Escherichia coli Chloramphenicol acetyltransferase;Escherichia coli CAT;CAT
Recombinant Proteins
- Human
- Bovine
- Rat
- Staphylococcus
- Mouse
- Chicken
- Zebrafish
- Bell pepper
- Dog
- E.coli
- Human Erythrocyte
- Bovine Liver
- Wheat Germ
- Mammalian Cell
- E. coli or Yeast
- Erythrocytes
- Insect Cells
- Insect Cell
- HEK293
- In Vitro Cell Free System
- HEK293T
- His
- Non
- GST
- His&Fc&Avi
- N-His&C-Myc
- His&Myc
- Myc&DDK
Involved Pathway
CAT involved in several pathways and played different roles in them. We selected most pathways CAT participated on our site, such as Tryptophan metabolism,Glyoxylate and dicarboxylate metabolism,Carbon metabolism, which may be useful for your reference. Also, other proteins which involved in the same pathway with CAT were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Carbon metabolism | ENO1A,FH,MTHFR,SUCLG2,SDHDA,GOT2B,EHHADH,SDHA,HADHA,GLUD1A |
| Glyoxylate and dicarboxylate metabolism | MDH2,MUT,MDH1,GLUL,HYI,ANI_1_2390104,GLULB,GCSH,DHDPSL,HAO2 |
| FoxO signaling pathway | TGFB1,G6PC2,SGK2A,PDPK1B,HOMER3,MAP2K2,PRKAB1,FOXO1B,RAG2,MAPK3 |
| Amyotrophic lateral sclerosis (ALS) | MAP3K5,CASP1,SLC1A2,DERL1,GRIN2B,MAPK13,BID,PPP3R1,GRIN1,GRIN2A |
| Tryptophan metabolism | HADHAA,AOX1,AANAT1,GCDHA,GCDHB,CCBL2,AOC1,ALDH2.2,AOX4,AADAT |
| Peroxisome | XDH,PEX5,NUDT12,DDO,PECR,ABCD3,BAAT,CRAT,MVK,ACSL1 |
Protein Function
CAT has several biochemical functions, for example, NADP binding,aminoacylase activity,antioxidant activity. Some of the functions are cooperated with other proteins, some of the functions could acted by CAT itself. We selected most functions CAT had, and list some proteins which have the same functions with CAT. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| antioxidant activity | MT3,SEPW1,TP53INP1,HP,KDM3B,PRDX5,UBIAD1,APOA4,TRP53INP1,SELS |
| heme binding | CYP17A1,CYGB,GUCY1A3,CYP20A1,CYP1D1,CYP19A1B,MB,CYP2D26,CYP2N13,CYP39A1 |
| catalase activity | GPX7,CYGB |
| enzyme binding | BTG1,TBP,HSPA1A,CTNNB1,MDM2,ATP6AP2,Slc4a2,POLB,DDC,SUPT5H |
| protein homodimerization activity | PRPS1,RBM44,BMP7A,S100A16,ASNSD1,KCNE2,HEXB,BNIP3,SDCBP,IZUMO3 |
| receptor binding | GIPC1,PTK2BB,UTS2,AGXT,DRD1A,CCL2,CAV1,RND1,PEX14,HTT |
| oxidoreductase activity, acting on peroxide as acceptor | PARK7 |
| metal ion binding | PRKCH,ZNF625,CYP46A1.1,NOX1,LOXL2B,EPT1,ZNF672,ZNF786,FTR82,DNAJC21 |
| NADP binding | NOS1,NOS2,CRYZL1,H6PD,FMO5,DPYD,ASPDH,HMGCRA,GSR,G6PDX |
Interacting Protein
CAT has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with CAT here. Most of them are supplied by our site. Hope this information will be useful for your research of CAT.
PTPN11;APP;APP;USP53;o68769_yerpe;p29991-pro_0000037946;KEAP1;proS;fcuA;leuB;15-deoxy-delta(12,14;midostaurin;JUP;SMARCA4;ACTR3;GNAS
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References
- Butz, E; Peichl, L; et al. Cone Bipolar Cells in the Retina of the Microbat Carollia perspicillata. JOURNAL OF COMPARATIVE NEUROLOGY 523:963-981(2015).
- Cong, W; Liu, GH; et al. Toxoplasma gondii infection in cancer patients: Prevalence, risk factors, genotypes and association with clinical diagnosis. CANCER LETTERS 359:307-313(2015).
