What is CHMP2A Protein

      CHMP2A, officially known as Charged Multivesicular Body Protein 2A, is a critical player in cellular processes with various synonyms such as Chromatin Modifying Protein 2A, VPS2-1, and hSnf7-1. Belonging to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family, CHMP2A plays a pivotal role in endosomal sorting and cellular membrane dynamics.

      This protein, characterized by its structural intricacies, is classified as a member of the AAA ATPase family and the ESCRT-III (endosomal sorting complex required for transport III) complex. Recent research advances have shed light on the nuanced functions and regulatory mechanisms of CHMP2A, expanding our understanding of cellular homeostasis.

      CHMP2A Biological Functions and Molecular Mechanisms

      The biological functions of CHMP2A are diverse and impactful. Primarily involved in the process of multivesicular body (MVB) formation, CHMP2A participates in the sorting and trafficking of cellular cargo destined for degradation. This is achieved through its role in the ESCRT-III complex, a molecular machinery responsible for the formation of intraluminal vesicles within endosomes, ultimately leading to the creation of MVBs.

      Molecular mechanisms underlying CHMP2A function include its interaction with other ESCRT-III components, orchestrating a finely-tuned process of cargo recognition, membrane deformation, and vesicle scission. This dynamic interplay ensures the proper removal of obsolete or damaged cellular components, contributing to cellular maintenance and homeostasis.

      Figure 1. The proposed mechanism of CHMP2A KO in tumor cells. (Bernareggi D, et al., 2022)

      Figure 1. The proposed mechanism of CHMP2A KO in tumor cells. (Bernareggi D, et al., 2022)

      CHMP2A Related Signaling Pathway

      The signaling pathways involving CHMP2A are intricately linked to endosomal sorting and membrane dynamics. As a core component of the ESCRT-III complex, CHMP2A is engaged in the downregulation of cell surface receptors, modulating their intracellular trafficking and subsequent degradation. This process is crucial for the regulation of various signaling pathways, impacting cellular responses to external stimuli.

      CHMP2A Related Diseases

      Anomalies in CHMP2A expression or function have been associated with various diseases, highlighting its significance in maintaining cellular health. Mutations in CHMP2A have been linked to neurodegenerative disorders, including frontotemporal dementia (FTD). Research suggests that dysregulation of the ESCRT machinery, including CHMP2A, may contribute to the pathological accumulation of protein aggregates seen in FTD and other related conditions.

      CHMP2A's Applications in Biomedicine

      The versatility of CHMP2A extends beyond its fundamental cellular functions, making it a promising candidate for biomedical applications. In diagnostic development, CHMP2A can serve as a potential biomarker for conditions associated with ESCRT dysfunction, aiding in the early detection and monitoring of diseases such as FTD.

      Furthermore, CHMP2A's involvement in cellular membrane dynamics positions it as a valuable player in vaccine development. Understanding its role in antigen presentation and endosomal processing provides a foundation for designing vaccines that harness the body's natural mechanisms for immune response, potentially enhancing vaccine efficacy.

      In the realm of therapeutics, the modulation of CHMP2A function presents a novel avenue for targeted interventions. Developing pharmaceuticals that regulate the ESCRT machinery could hold promise for conditions where aberrant cellular clearance contributes to pathology, such as neurodegenerative diseases.

      Recommended Products

      Cat.# Product name Species Source (Host) Tag
      CHMP2A-4011H Recombinant Human CHMP2A Protein, Myc/DDK-tagged, C13 and N15-labeled Human HEK293T Myc/DDK
      CHMP2A-11184H Recombinant Human CHMP2A, GST-tagged Human E.coli GST
      CHMP2A-1396H Recombinant Human Chromatin Modifying Protein 2A, His-tagged Human E.coli His
      CHMP2A-3205HF Recombinant Full Length Human CHMP2A Protein, GST-tagged Human In Vitro Cell Free System GST
      CHMP2A-26719TH Recombinant Human CHMP2A, His-tagged Human E.coli His
      CHMP2A-27958TH Recombinant Human CHMP2A, His-tagged Human E.coli His
      CHMP2A-1249H Recombinant Human CHMP2A Protein, GST-Tagged Human Wheat Germ GST
      CHMP2A-5210H Recombinant Human CHMP2A protein, GST-tagged Human E.coli GST
      Chmp2a-2146M Recombinant Mouse Chmp2a Protein, Myc/DDK-tagged Mouse HEK293T Myc/DDK
      CHMP2A-1650M Recombinant Mouse CHMP2A Protein, His (Fc)-Avi-tagged Mouse HEK293 His (Fc)-Avi

      Reference

      • Bernareggi D, et al. CHMP2A regulates tumor sensitivity to natural killer cell-mediated cytotoxicity. Nature Communications. 2022, 13(1): 1899.