IVD of Hepatitis C Virus

Hepatitis C Virus (HCV)

Hepatitis C is a viral hepatitis caused by hepatitis C virus (HCV) infection. It is mainly transmitted through blood. Hepatitis C is a global epidemic and can lead to chronic inflammation and necrosis of the liver, and even develop into Liver cirrhosis or hepatocellular carcinoma are extremely harmful to human health and life and have become serious social and public health problems. HCV infection is primarily determined clinically by measuring hepatitis C core antigens (HCV-cAgs) and hepatitis C antibodies (HCV-Abs).

HCV-related mechanisms of carcinogenesis: from HCV infection to HCC.
Figure 1. HCV-related mechanisms of carcinogenesis: from HCV infection to HCC. (Vescovo T, et al., 2016)

Main Methods of IVD for Hepatitis C Virus

In vitro diagnostic (IVD) methods for HCV mainly rely on biochemistry and molecular biology techniques.

  • HCV antibody and antigen test. Antibody testing can determine whether patients have been infected with HCV by detecting HCV-Ab or HCV-cAg levels in the serum.
  • HCV RNA testing. HCV genetic material, HCV-RNA, can be detected in blood by RNA testing for HCV infection.
  • Polymerase chain reaction (PCR) technology is used to amplify and detect the HCV gene sequence using specific probes.

Creative BioMart can provide high-quality recombinant HCV core proteins used for IVD, including ELISA, lateral flow assays, western blots, and other immunoassays.

Highlights of Our Products

  • High sensitivity, high specificity, and high purity.
  • It is widely used and suitable for downstream immunological experiments.
  • Easy to store and transport, conducive to large-scale production and use of vaccines.
  • Outstanding success rate and fast development speed.

Our Outstanding Advantages

  • IVD proteins can be used to test for a variety of diseases and conditions, making them valuable tools for diagnosing and monitoring health.
  • Guarantee high performance, high reliability, and high consistency of protein quality, leading the industry.
  • A complete IVD protein platform can provide customized services to meet different scientific research needs.
  • High-quality service, high-level experiments, and reliable analysis.

In addition, Creative BioMart also offers a series of viral proteins and protein-related services to provide customers with high-quality, low-cost active recombinant proteins to meet different needs and assist in preclinical drug development.

Creative BioMart offers a series of highly efficient and reliable IVD proteins, providing strong support for medical research and preclinical diagnosis. At the same time, we provide more flexible and personalized protein services that are used in IVD reagents, drug development, biotechnology research and production, and other fields. Please contact us for answers if you are interested in our products or services.

Case Study

Case 1: Youssef SS, Elemeery MN, Eldein SS, Ghareeb DA. Silencing HCV Replication in Its Reservoir. Open Access Maced J Med Sci. 2018 Nov 16;6(11):1965-1971. doi: 10.3889/oamjms.2018.372. PMID: 30559844; PMCID: PMC6290455.

This study aims to test the efficacy of siRNA against HCV-4 replication in PBMC in vitro, to introduce an alternative therapeutic option for HCV-4 suitable to eradicate it from both hepatic and extra-hepatic reservoirs.

The research concluded that targeting HCV infection using RNA interference technology might be a reliable therapeutic option for chronic HCV patients with HCV minus strand within PBMCs.

Fig2. Composite picture for Western blot for HCV-NS5A compared to intracellular GAPDH

Case 2: Wang Y, Jie W, Ling J, Yuanshuai H. HCV core antigen plays an important role in the fight against HCV as an alternative to HCV-RNA detection. J Clin Lab Anal. 2021 Jun;35(6):e23755. doi: 10.1002/jcla.23755. Epub 2021 Mar 31. PMID: 33788295; PMCID: PMC8183919.

To discuss the clinical significance of HCV-cAg testing in the diagnosis, activity determination, and monitoring of therapeutic effectiveness of HCV infection and its advantages compared with HCV-RNA and anti-HCV antibodies detection.

HCV core antigen has similar clinical sensitivity to NAT and can be used as a substitute for HCV-RNA in the diagnosis of virus infection. Combined detection of HCV-cAg and antibody serology can help doctors detect HCV infection earlier, accurately diagnose different stages of HCV infection, and evaluate the therapeutic effect of antiviral drugs, which are beneficial in the prevention and treatment of hepatitis C.

Fig3. Emergence of laboratory markers for HCV infection. This image describes the time point at which various markers of infection (HCV-RNA, HCV-CAG, and anti-HCV) first appeared in the body of an HCV infection

Reference

  • Vescovo T, Refolo G, Vitagliano G, et al. (2016). Molecular mechanisms of hepatitis C virus–induced hepatocellular carcinoma[J]. Clinical Microbiology and Infection. 22(10): 853-861.