PPP2R5A
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Official Full Name
protein phosphatase 2, regulatory subunit B, alpha -
Overview
The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. -
Synonyms
PPP2R5A;protein phosphatase 2, regulatory subunit B, alpha;protein phosphatase 2, regulatory subunit B (B56), alpha isoform , protein phosphatase 2, regulatory subunit B, alpha isoform;serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit alpha isoform;B56A;PR61A;2A5A_HUMAN;MGC131915;PP2A B Subunit B Alpha Isoform;PP2A B Subunit B56 Alpha Isoform;PP2A B subunit isoform B-alpha;PP2A B subunit isoform B56-alpha;PP2A B subunit isoform PR61-alpha;PP2A B subunit isoform R5-alpha;PP2A B Subunit PR61 Alpha Isoform;PP2A B Subunit R5 Alpha Isoform;PR61alpha;Protein phosphatase 2 regulatory subunit B (B56) alpha isoform;Serine/Threonine Protein Phosphatase 2A 56kDa Regulatory Subunit Alpha Isoform;OTTHUMP00000034891;PP2A, B subunit, B alpha isoform;PP2A, B subunit, R5 alpha isoform;PP2A, B subunit, B56 alpha isoform;PP2A, B subunit, PR61 alpha isoform;protein phosphatase 2, regulatory subunit B, alpha isoform;protein phosphatase 2, regulatory subunit B (B56), alpha isoform;serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, alpha isoform
Recombinant Proteins
- Human
- Mouse
- Rhesus macaque
- E.coli
- Mammalian Cells
- Wheat Germ
- HEK293
- In Vitro Cell Free System
- GST
- His
- Non
- Avi
- Fc
| Cat.# | Product name | Source (Host) | Species | Tag | Protein Length | Price |
|---|---|---|---|---|---|---|
| PPP2R5A-1919H | Recombinant Human PPP2R5A, GST-tagged | E.coli | Human | GST | C-term-299aa | |
| PPP2R5A-13261M | Recombinant Mouse PPP2R5A Protein | Mammalian Cells | Mouse | His |
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| PPP2R5A-31104TH | Recombinant Human PPP2R5A | Wheat Germ | Human | Non | 486 amino acids |
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| PPP2R5A-3574R | Recombinant Rhesus monkey PPP2R5A Protein, His-tagged | Mammalian Cells | Rhesus macaque | His |
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| PPP2R5A-1404HCL | Recombinant Human PPP2R5A cell lysate | Human | Non |
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| PPP2R5A-3392R | Recombinant Rhesus Macaque PPP2R5A Protein, His (Fc)-Avi-tagged | HEK293 | Rhesus macaque | Avi&Fc&His |
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| PPP2R5A-3392R-B | Recombinant Rhesus Macaque PPP2R5A Protein Pre-coupled Magnetic Beads | HEK293 | Rhesus macaque |
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| PPP2R5A-394HF | Recombinant Full Length Human PPP2R5A Protein | In Vitro Cell Free System | Human | Full L. 486 amino acids |
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| PPP2R5A-7038M | Recombinant Mouse PPP2R5A Protein, His (Fc)-Avi-tagged | HEK293 | Mouse | Avi&Fc&His |
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| PPP2R5A-7038M-B | Recombinant Mouse PPP2R5A Protein Pre-coupled Magnetic Beads | HEK293 | Mouse |
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Background
What is PPP2R5A Protein?
PPP2R5A is a part of the enzyme known as protein phosphatase 2A (PP2A), which is crucial for controlling cell growth and communication. This subunit influences which proteins PP2A targets, affecting various signaling pathways in the cell. Because it plays a role in processes like cell division and signal regulation, any changes in PPP2R5A can be linked to diseases like cancer. Researchers are particularly interested in this protein when studying how cells cycle and how we might target it for cancer treatments. Keywords like "cancer pathways" and "drug targets" often come up when discussing PPP2R5A in the context of scientific research.
Fig1. PP2A is a family of serine/threonine phosphatases. (Terrance J Haanen 3rd, 2022)
What is the Function of PPP2R5A Protein?
PPP2R5A acts as a regulatory piece of the PP2A enzyme, which is vital for controlling how cells signal and grow. It helps decide which proteins get dephosphorylated by PP2A, thus influencing the enzyme's actions in the cell. This role is important for processes like cell division and programmed death. If PPP2R5A isn't functioning right, it can throw off cell signaling, which is a concern in cancer and other diseases. Researchers look at PPP2R5A in studies related to "cell signaling" and "cancer treatment," considering its potential in fixing signaling issues to treat disease better.PPP2R5A Related Signaling Pathway
PPP2R5A is an essential yet low-key protein involved in managing how cells grow and divide, playing a significant role in processes like cancer prevention. It's part of the phosphatase 2A regulatory subunit B family and interacts with proteins such as AXIN1 and CTLA-4, which are crucial for the Wnt signaling pathway that governs cell fate and regeneration. The gene for PPP2R5A is located on chromosome 1 and is highly active in tissues like skeletal muscles and bone marrow. Understanding its role helps uncover new therapeutic targets for diseases with disrupted cell regulation.PPP2R5A Related Diseases
PPP2R5A is linked to several diseases due to its role as a regulatory protein. When this gene doesn't function properly, it can disrupt how our cells grow and divide, often leading to a higher risk of cancer. Since it interacts with key signaling proteins and pathways, any mutation or dysfunction might contribute to not only cancer but also neurodegenerative conditions and other disorders involving cell regulation. Understanding PPP2R5A's impacts might help us develop targeted therapies for diseases that stem from these cellular miscommunications.Bioapplications of PPP2R5A
PPP2R5A holds potential for diverse bioapplications due to its role in cell regulation. In the field of cancer research, targeting PPP2R5A could aid in developing therapies that prevent unchecked cell division. Its involvement in signaling pathways makes it a candidate for studying neurodegenerative diseases, where cell communication goes awry. Additionally, PPP2R5A's ability to interact with various proteins might be harnessed to design precision medicine treatments, where specific pathway disruptions can be corrected. Researchers are diving into its functions to potentially apply these findings in regenerative medicine, highlighting the exciting possibilities PPP2R5A offers in advancing medical science.Case Study
Case Study 1: Leonard D. et al. Cell. 2020
Protein phosphatases like PP2A are key in diseases such as cancer. They control dephosphorylation, and PP2A's function is guided by regulatory subunits. Researchers found that DT-061 stabilizes the B56α-PP2A holoenzyme, keeping it active to target proteins like c-Myc. This research uncovered how DT-061 binds to PP2A components, preventing disassembly. This discovery highlights a new method for stabilizing PP2A, offering a path for phosphatase-targeted treatments.-
Fig1. Co-IP of V5-tagged PP2A-A from xenograft tumors treated for 29 days with DMSO or DT-061 followed by mass spectrometry and SAINT analysis of co-immunoprecipitated proteins. -
Fig2. Percent of L309 methylation of the PP2A catalytic subunit bound to WT-A or R183W-A holoenzymes in cell culture as determined by mass spectrometry.
Case Study 2: Zhuang Z. et al. Oncol Rep. 2017
Research shows that microRNAs (miRNAs) are key in how tumor cells respond to chemotherapy. In this study, researchers found that miR-218 is upregulated in cisplatin-resistant oral cancer cells. When miR-218 is high, cells survive better and resist cisplatin, but reducing miR-218 triggers apoptosis and increases drug sensitivity. They identified PPP2R5A as a direct target of miR-218. Boosting miR-218 reduces PPP2R5A, while lowering miR-218 raises its levels. Adding PPP2R5A counteracts miR-218's effects on survival and drug resistance. Knocking down β-catenin or overexpressing PPP2R5A curbs miR-218's Wnt activation, restoring sensitivity to cisplatin.-
Fig3. UM1 and Cal27 cells transfected with miR-218, PPP2R5A or control were treated with cisplatin (2.5 µg/ml). -
Fig4. Lysates from UM1 and UM1cis cells after transfection for 48 h with miR-218 or anti-miR-218 were analyzed by western blotting to evaluate PPP2R5A expression.
Involved Pathway
PPP2R5A involved in several pathways and played different roles in them. We selected most pathways PPP2R5A participated on our site, such as mRNA surveillance pathway,Sphingolipid signaling pathway,Oocyte meiosis, which may be useful for your reference. Also, other proteins which involved in the same pathway with PPP2R5A were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Adrenergic signaling in cardiomyocytes | MYL3,ADRB2,CAMK2B,ATP1B2A,TNNT2,PRKACAA,PLCB1,CALM,CACNA1D,MAPK11 |
| PIK-Akt signaling pathway | PPP2R5B,GNG5,FGF7,INS1,LAMA1,IFNA8,Fgf15,TCL1,PPP2R2D,RPS6KB1 |
| Oocyte meiosis | SMC1A,CCNB2,CDC27,CDC25C,PPP3CCA,ANAPC4,CALM,RPS6KA3B,IGF1RA,PPP2CB |
| Sphingolipid signaling pathway | MAP2K1,SGMS2,PPP2R5B,PPP2R2B,PIK3R1,SPHK2,HRAS,PLCB4,PIK3CA,RHOA |
| mRNA surveillance pathway | PABPC3,CPSF5,PPP2R5E,PPP2R2CA,PABPC1B,PPP1CAB,PPP2R2A,PABPC4,ETF1A,GSPT1 |
| Dopaminergic synapse | CAMK2B,MAPK11,PRKACB,MAPK9,DRD2,GNG3,KCNJ3,SCN1A,PPP2R2B,PPP2R2D |
| AMPK signaling pathway | FOXO1,IRS2,PFKP,CAMKK1,PIK3R3,IRS4,PPP2R1B,RAB10,AKT2,GYS1 |
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Fig1. Schematic Depicting Effects of DT-061 on Protein Phosphatase 2A (PP2A) and Its Regulation of c-MYC. (Vidhi M Shah, 2020) -
Fig2. The diagrammatic drawing of PPP2R5A molecular structure and its related signal pathways. (Ziming Mao, 2018)
Protein Function
PPP2R5A has several biochemical functions, for example, kinase binding,protein binding,protein phosphatase type 2A regulator activity. Some of the functions are cooperated with other proteins, some of the functions could acted by PPP2R5A itself. We selected most functions PPP2R5A had, and list some proteins which have the same functions with PPP2R5A. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| kinase binding | WWC2,WWC1,SRC,ATF5,TRIP6,C2orf44,RPS3,TENC1,CAB39,DLG1 |
| protein phosphatase type 2A regulator activity | PPP2R2BB,PPP2R5E,PPP2R4,ENSA,PPP2R5B,ARPP19,PPP2R1A,PPP2R2A,PPME1,PPP2R2B |
| protein binding | FAM113B,IFI16,ACVR2B,PCID2,ADAMTSL5,HYLS1,DEPDC1,BCL2A1,FGD2,RBM22 |
Interacting Protein
PPP2R5A has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with PPP2R5A here. Most of them are supplied by our site. Hope this information will be useful for your research of PPP2R5A.
PPP2CA;PPP2R1A;PPP2R1B;Ppp2r1a;Sgol2;SGOL1;CHEK2
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