GCLC
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Official Full Name
glutamate-cysteine ligase, catalytic subunit -
Overview
Gamma Glutamylcystuene Synthetase (GSH) is the first enzyme and rate limiting step in the slutathione biosynthesis pathway. Glutathione (GSH) plays an important role in detoxification of oxidants and toxins from the cells. In most cells, GSH is synthesized denovo in two steps catalysed by Gamma Glutamylcysteine Synthetase (GCS) / Glutamate-cystein Ligase and GSH homeostasis. It is composed of two subunits: heavy and light. These subunits are coded by different genes which are controlled by different mechanisms. The heavy subunit carries the catalytic activity and can be inhibited through feedback mechanism by GSH. Binding of light subunit to heavy subunit reduces the Michaelis-Menten constant for glutamate to a physiological concentration of 1.2mM and increases the inhibitory constant for GSH. -
Synonyms
GCLC;glutamate-cysteine ligase, catalytic subunit;GLCL, GLCLC;glutamate--cysteine ligase catalytic subunit;GCS;gamma-ECS;GCS heavy chain;gamma-glutamylcysteine synthetase;GCL;GLCL;GLCLC
Recombinant Proteins
- Rhesus macaque
- Rat
- Human
- Mouse
- Zebrafish
- Mammalian Cells
- Wheat Germ
- HEK293
- E.coli
- In Vitro Cell Free System
- His
- Non
- Myc&DDK
- His&Fc&Avi
- GST
- Flag
Involved Pathway
GCLC involved in several pathways and played different roles in them. We selected most pathways GCLC participated on our site, such as Cysteine and methionine metabolism,Glutathione metabolism,Metabolic pathways, which may be useful for your reference. Also, other proteins which involved in the same pathway with GCLC were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| Cysteine and methionine metabolism | MAT2AA,CBSA,AMD1,DNMT3,SDSL,GOT1,MDH1AA,LDHA,MAT1A,TAT |
| Metabolic pathways | NME6,PPT2,ACAD8,ARG1,KLF15,GALM,NOS2A,ATP6V0A1B,POLR3B,UPP1 |
| Glutathione metabolism | GSTA4,GGT7,MGST3,GSTA5,GSTA1,GSTA,GSR,GPX5,GGT1,ANPEPB |
Protein Function
GCLC has several biochemical functions, for example, ADP binding,ATP binding,coenzyme binding. Some of the functions are cooperated with other proteins, some of the functions could acted by GCLC itself. We selected most functions GCLC had, and list some proteins which have the same functions with GCLC. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| protein heterodimerization activity | BNIP3,SUPT3H,TCF12,DMRT3,SNX2,PIK3R2,TAF11,CEBPE,MAP3K7,INHA |
| glutamate binding | GRIN1,SLC1A1,GAD2,GRIK1,CPS1,GRIN2A,GLUL,SLC1A3,GRM7 |
| coenzyme binding | NDUFA9,GCH1,HMGCRB,ACAT1,TDH,WWOX,TDH2,HMGCR,HMGCRA,NDUFA9A |
| ATP binding | CCT7,BMPR1BA,CAMK2A,DHX34,CAMK2G1,KCNJ11,VMHCL,KIF23,SNRNP200,PKZ |
| magnesium ion binding | RRAGC,ENOSF1,FOXK2,FAN1,ATP8B3,RPS6KA6,EYA2,COMT,HMGCL,TESC |
| ADP binding | MYH10,ME1,MYO18A,CHORDC1,PRPS2,PRKAG2,MYO9B,GLUD1,APAF1,MYO6 |
Interacting Protein
GCLC has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with GCLC here. Most of them are supplied by our site. Hope this information will be useful for your research of GCLC.
CCL22;dacA
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References
- Sueblinvong, V; Tseng, V; et al. TGF beta 1 Mediates Alcohol-Induced Nrf2 Suppression in Lung Fibroblasts. ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH 38:2731-2742(2014).
- Wisniewski, M; Lapinski, M; et al. Molecular cloning and analysis of Ancylostoma ceylanicum glutamate-cysteine ligase. MOLECULAR AND BIOCHEMICAL PARASITOLOGY 196:12-20(2014).
