Principle & Protocol

The purpose of this experiment manual is to help scientific researchers master the preparation of Arabidopsis protoplasts, learn the technology of detecting protein-protein interaction using protein subcellular co-localization method, and be familiar with the operation process and precautions of co-transfection technology.

The laboratory manual is designed to help researchers master the principles of immunoprecipitation and its applications, learn to apply immunoprecipitation methods to detect protein interactions, and become familiar with the operational procedures and precautions for immunoprecipitation techniques.

The purpose of this experiment manual is to help scientific researchers master the principle and application of the yeast two-hybrid Sos recruited system, learn to use the co-transformation method to screen the interacting proteins and be familiar with the operation process and precautions of the yeast two-hybrid system for library screening.

The purpose of this experimental protocol is to help researchers to grasp the principle and application of the classic yeast two hybrid system, learn the methods of preparation and transformation of yeast cell competent state, and be familiar with the operation process and experimental precautions of yeast two hybrid system for detecting protein interaction.

The purpose of this experimental protocol is to help researchers to understand the basic principle of purification of maltose binding protein (MBP) fusion protein. Master the expression and purification methods and main operation steps of MBP fusion protein.

The purpose of this experimental protocol is to help researchers to understand the basic principle of fusion protein expression and purification. Master the expression and purification methods of histidine labeled fusion protein, as well as the main operation steps and precautions.

The purpose of this experimental protocol is to help researchers to understand the basic principle of fusion protein expression and purification. Master the expression and purification methods of glutathione S-transferase (GST) fusion protein, as well as the main operation steps and precautions.

The purpose of this experimental protocol is to help researchers mastering the basic principles and main steps of prokaryotic expression of the target protein, and master the basic steps and precautions of Coomassie brilliant blue staining and immunoblotting experiment.

Silver staining is an important dyeing method, which is widely used because of its low cost, safe reagents, fast and simple, and high sensitivity. The purpose of this experimental protocol is to help researchers learn the principle, technology and application of Silver Staining.

Coomassie brilliant blue staining method can reach the detection level of microgram level, and the linear dynamic correlation range between staining degree and protein content is wide, which is suitable for quantitative analysis. The purpose of this experimental protocol is to help researchers learn the principle, technology and application of Coomassie brilliant blue dyeing.