Principle & Protocol
Principle and Protocol of Isotope Labeling
The purpose of this experiment guide is to help researchers master the basic principles of iTRAQ technology and understand the experimental process and precautions of iTRAQ.
Principle and Protocol of Fluorescence Labeled Differential Analysis Gel Electrophoresis
The purpose of this experiment manual is to help researchers master the basic principles and main steps of using two-dimensional electrophoresis combined with fluorescent dye labeling to analyze differentially expressed proteins and the method of using DeCyder software to analyze differentially expressed proteins.
Guide of Analysis of Differentially Expressed Proteins by Two-dimensional Electrophoresis
This experimental manual focuses on the main methods of two-dimensional electrophoresis, the analysis methods of differential proteins and the optimization of parameters.
Guide of PCR-assisted Analysis of Protein-DNA Sequence Specificity
The main purpose of this experimental manual is to help scientific researchers understand the principle of PCR-assisted protein-DNA sequence specificity analysis and master the main operating steps and precautions of PCR-assisted protein-DNA sequence specificity analysis.
Principle and Protocol of Yeast One-hybrid Analysis
The purpose of this experiment manual is to help scientific researchers understand the basic principle and application of yeast single hybridization, master the main steps and precautions of yeast single hybridization, learn the production and transformation of yeast receptive state, and the construction and screening of gene library.
Guide of Analysis of Transient Cell Interaction
The purpose of this experiment manual is to help scientific researchers understand the principle of cell transient interaction analysis and master the main operating steps and precautions of cell transient interaction analysis.
Principle and Protocol of ChIP
The purpose of this lab manual is to help researchers master the screening of DNA sequences interacting with target proteins (regulators), to obtain information on protein-DNA interactions, and to master the principles, applications, and major steps of ChIP.
Principle and Protocol of EMSA
The purpose of this laboratory manual is to help researchers understand the principles of electrophoretic mobility analysis techniques and to master the main operational steps and precautions for gel migration.
Guide of Analysis of Promoter Regulatory Elements Based on Plant CARE Prediction Method
The purpose of this experiment manual is to help scientific researchers master the PlantCARE database software of plant cis-acting elements, and analyze the promoter regulatory elements through the PlantCARE online prediction method.
Principle and Protocol of Pull-down Technology
The purpose of this experiment manual is to help scientific researchers learn the experimental principle of pull-down technology to identify protein interaction, and be familiar with the operation process and precautions of MBP pulldown technology.