What is FASTK Protein

      FASTK, or Fas-activated serine/threonine kinase, is a crucial protein that plays a pivotal role in cellular processes. Also known as FASTKD1, it is encoded by the FASTKD1 gene. Synonyms for FASTK include FAST kinase domain-containing protein 1 and Fas-activated serine/threonine kinase domain-containing protein 1.

      FASTK Protein Structural Characteristics and Classification

      Belonging to the FAST kinase domain-containing (FASTKD) protein family, FASTK is essential for maintaining mitochondrial function. These proteins are characterized by a kinase domain and RNA-binding motifs. FASTK, in particular, possesses a unique combination of domains, including a K-homology (KH) domain and a RAP domain. This structural makeup underscores its significance in RNA metabolism and cellular homeostasis.

      Recent Research Advances about FASTK Protein

      Recent research has shed light on the multifaceted roles of FASTK within the cell. Studies have highlighted its involvement in the regulation of mitochondrial RNA processing, specifically in the maturation of mitochondrial tRNAs. Moreover, FASTK has been implicated in the maintenance of mitochondrial DNA integrity, linking it to the overall health of the cell.

      FASTK Biological Functions and Molecular Mechanisms

      FASTK's biological functions are intricately tied to its role in mitochondrial RNA processing and metabolism. It acts as a key player in the maturation of mitochondrial tRNAs, ensuring their proper functionality in the translation machinery. Additionally, FASTK has been implicated in the regulation of mitochondrial gene expression, impacting the overall energy production and cellular respiration.

      At the molecular level, FASTK exerts its influence through RNA-binding interactions. Its KH domain facilitates binding to specific RNA sequences, allowing it to participate in the processing and stabilization of mitochondrial RNAs. The RAP domain, on the other hand, is involved in protein-protein interactions, contributing to the formation of complexes that modulate mitochondrial gene expression.

      Figure 1. Oxidative stress-induced FASTK mRNA degradation in alcoholic cardiomyopathy. (Zhang F, et al., 2021)

      Figure 1. Oxidative stress-induced FASTK mRNA degradation in alcoholic cardiomyopathy. (Zhang F, et al., 2021)

      FASTK Related Signaling Pathway

      The signaling pathways involving FASTK are complex and interconnected. Notably, its association with mitochondrial function implicates it in pathways related to cellular energetics and stress responses. Research suggests that FASTK may respond to cellular stress signals, modulating its activity to ensure the maintenance of mitochondrial integrity and function.

      FASTK Related Diseases

      Understanding the implications of FASTK dysregulation is critical in deciphering its connection to various diseases. Recent research has identified links between FASTK and mitochondrial disorders, particularly those affecting energy metabolism. Dysfunctional FASTK has been associated with disruptions in mitochondrial RNA processing, leading to defects in mitochondrial translation and energy production.

      FASTK's Applications in Biomedicine

      • Diagnostic Development

      Given its central role in mitochondrial RNA processing, FASTK holds promise as a diagnostic marker for mitochondrial disorders. Detection of aberrant FASTK levels or activity could serve as an indicator of mitochondrial dysfunction, aiding in the early diagnosis of related diseases.

      • Vaccine Development

      Understanding the molecular mechanisms governed by FASTK provides a basis for exploring its potential in vaccine development. Targeting FASTK-related pathways could offer new avenues for designing vaccines aimed at bolstering mitochondrial function and cellular resilience.

      • Therapeutics

      The intricate involvement of FASTK in cellular processes makes it an attractive target for therapeutic interventions. Modulating FASTK activity could potentially ameliorate mitochondrial disorders and enhance cellular function. Small molecules or gene therapies targeting FASTK-related pathways may emerge as novel therapeutic strategies in the future.

      Recommended Products

      Cat.No. Product Name Species Source (Host) Tag
      FASTK-1402H Active Recombinant Human FASTK (Active), GST-tagged Human Sf9 Insect Cell GST
      FASTK-801H Recombinant Human FASTK, GST-tagged Human Sf9 Insect Cell GST
      FASTK-824H Recombinant Human Fas-activated Serine/Threonine Kinase, T7-tagged Human E.coli T7
      FASTK-3859H Recombinant Human FASTK Protein, GST-tagged Human Wheat Germ GST
      FASTK-3315H Recombinant Human FASTK Protein, Myc/DDK-tagged, C13 and N15-labeled Human HEK293T Myc/DDK
      FASTK-02H Recombinant Human FASTK Protein, Myc/DDK-tagged Human HEK293T Myc/DDK
      FASTK-721H Recombinant Human FASTK Protein, His-tagged Human E.coli His
      FASTK-01H Recombinant Human FASTK Protein, His-tagged Human E.coli His
      FASTK-4863HF Recombinant Full Length Human FASTK Protein, GST-tagged Human In Vitro Cell Free System GST
      Fastk-2957M Recombinant Mouse Fastk Protein, Myc/DDK-tagged Mouse HEK293T Myc/DDK

      Reference

      • Zhang F, et al. Accelerated FASTK mRNA degradation induced by oxidative stress is responsible for the destroyed myocardial mitochondrial gene expression and respiratory function in alcoholic cardiomyopathy. Redox Biology. 2021, 38: 101778.