IVD of Rubella Virus

Cat. #	Product name	Source (Host)	Species	Tag	Protein Length	Price
E1-274V	Active Recombinant Rubella Virus E1 mosaic Protein, His-tagged	E.coli	RUBV	His	157-176;213-239;374-390 a.a.	Inquiry
C-472V	Recombinant Rubella Virus Capsid (C) Protein	E.coli	RUBV	Non	1-123 a.a.	Inquiry
Capsid-422V	Recombinant Rubella Capsid C Protein	E.coli	RUBV	Non	1-123 a.a.	Inquiry
Capsid-757R	Recombinant Rubella Virus Capsid Protein	E.coli	RUBV	Non	1-123 a.a.	Inquiry
E1-420V	Recombinant Rubella E1 Protein, His-tagged	E.coli	RUBV	His	157-276 a.a.	Inquiry
E1-473V	Recombinant Rubella Virus E1 mosaic Protein	E.coli	RUBV	Non	157-176;374-390;213-239 a.a.	Inquiry
E2-273V	Recombinant Rubella Virus E2 Protein, His-tagged	E.coli	RUBV	His	31-105 a.a.	Inquiry
E2-421V	Recombinant Rubella E2 Protein, His-tagged	E.coli	RUBV	His	31-105 a.a.	Inquiry
E2-474V	Recombinant Rubella Virus E2 Protein	E.coli	RUBV	Non	31-105 a.a.	Inquiry
Envelope-756R	Recombinant Rubella Virus Envelope GlycoProtein E2 Protein	E.coli	RUBV	Non	31-105 a.a.	Inquiry
Mosaic-755R	Recombinant Rubella Virus GlycoProtein E1 Mosaic Protein	E.coli	RUBV	Non	157-176;213-239;374-390 a.a.	Inquiry
N-304V	Recombinant Rubella Nucleocapsid Protein	E.coli	RUBV	Non		Inquiry
N-305V	Recombinant Rubella Nucleocapsid Protein	Insect Cells	RUBV	Non		Inquiry
E1/E2-25R	Recombinant Rubella virus E1-E2 Protein					Inquiry
E1-24R	Recombinant Rubella virus E1 Protein					Inquiry

Rubella Virus (RV)

Rubella virus (RV) is an RNA virus limited to humans, which can cause rubella, an acute respiratory infectious disease, after infecting the human body. Patients with RV infection develop complications such as fever, rash, arthritis, and swollen lymph nodes behind the ear. RV is prone to vertical infection. Early pregnancy infection with rubella can cause miscarriage or malformation, as well as congenital rubella syndrome (CRS) in the fetus. Currently, the main means of preventing RV infection is planned vaccination.

Main Steps of IVD for Rubella Virus

Antibody testing. Rubella can be diagnosed by detecting IgM of RV antibodies in the patient's blood. In addition, RV antibody IgM can also be detected in pregnant women's amniotic fluid or umbilical cord blood. Once the test result is positive, the fetus can be diagnosed with RV infection.
Virological examination. RV is isolated by taking the patient's throat swab specimens or urine specimens. If the RV is isolated, the diagnosis can be confirmed.
Blood test. The decrease in the total number of white blood cells and the increase in the proportion of lymphocytes in the patient's serum is helpful for the diagnosis of RV infection.
Use molecular biology techniques, such as gene chips and RT-PCR to detect the genetic sequence of the virus to determine the genotype and subtype of the RV.

Creative BioMart provides high-quality recombinant RV proteins used for IVD, including ELISA, lateral flow assays, western blots, and other immunoassays.

Highlights of Our Products

High sensitivity, high specificity, and high purity.
A wide range of immune tests are available, such as ELISA, lateral flow, WB, and others.
Easy to store and transport, conducive to large-scale production and use of vaccines.
Outstanding success rate and fast development speed.

Our Outstanding Advantages

IVD proteins can be used to test for a variety of diseases and conditions, making them valuable tools for diagnosing and monitoring health.
Guarantee high performance, high reliability, and high consistency of protein quality, leading the industry.
A complete IVD protein platform can provide customized services to meet different scientific research needs.
High-quality service, high-level experiments, and reliable analysis.

Applications

Vaccine Development: Rubella virus proteins, particularly the envelope glycoproteins E1 and E2, are crucial components of the rubella vaccine. These proteins elicit an immune response that helps protect against rubella infection. The live attenuated rubella vaccine, often combined with measles and mumps vaccines (MMR), has been highly effective in preventing rubella.

Diagnostics: Rubella virus proteins are used in serological assays to diagnose rubella infection. Tests such as enzyme-linked immunosorbent assays (ELISAs) use these proteins to detect antibodies (IgM and IgG) in a patient's blood, indicating current or past infection or vaccination status.

Research: In virology research, Rubella virus proteins are studied to understand the virus's structure, replication, and pathogenesis better. This research can lead to improved diagnostic methods, treatments, and vaccines.

Therapeutic Research: Rubella virus proteins are being studied for potential therapeutic applications, including as vectors for delivering therapeutic genes in gene therapy.

Public Health: Monitoring the prevalence of rubella virus antibodies in populations help public health officials assess the effectiveness of vaccination programs and the need for additional public health interventions.

Pregnancy Monitoring: Pregnant women are often tested for rubella immunity, as a rubella infection during pregnancy can lead to congenital rubella syndrome (CRS), causing severe birth defects. Monitoring Rubella virus antibody levels helps in managing and preventing CRS.

Case Study

Case 1: Das PK, Gonzalez PA, Jangra RK, Yin P, Kielian M. A single-point mutation in the rubella virus E1 glycoprotein promotes rescue of recombinant vesicular stomatitis virus. mBio. 2024 Mar 13;15(3):e0237323. doi: 10.1128/mbio.02373-23. Epub 2024 Feb 9. PMID: 38334805; PMCID: PMC10936182.

Rubella virus (RuV) is an enveloped plus-sense RNA virus and a member of the Rubivirus genus. RuV infection in pregnant women can lead to miscarriage or an array of severe birth defects known as congenital rubella syndrome. To promote the study of rubivirus biology, in this article, the authors generated replication-competent recombinant VSV-RuV (rVSV-RuV) encoding the RuV transmembrane glycoproteins E2 and E1. Sequencing of rVSV-RuV showed that the RuV glycoproteins acquired a single-point mutation W448R in the E1 transmembrane domain.

Fig2. Effects of E1 W448R on the properties of expressed RuV E2/E1. Cells were tested for E2/E1 glycosylation. Lysates were treated with no enzyme (NE), PNGaseF (F), or EndoH (H) and analyzed by WB with RuV pAb (left panel) or E2 mAb (right panel). PNGaseF-sensitive and EndoH-resistant forms of E1 and E2 are marked by blue or magenta asterisks, respectively.

Case 2: Yagi M, Hama M, Ichii S, Nakashima Y, Kanbayashi D, Kurata T, Yusa K, Komano J. Sphingomyelin synthase 1 supports two steps of rubella virus life cycle. iScience. 2023 Oct 26;26(11):108267. doi: 10.1016/j.isci.2023.108267. PMID: 38026182; PMCID: PMC10654604.

Our knowledge of the regulatory mechanisms that govern the replication of the rubella virus (RV) in human cells is limited. To gain insight into the host-pathogen interaction, researchers conducted a loss-of-function screening using the CRISPR-Cas9 system in the human placenta-derived JAR cells.

Fig 3. RV infection in cells expressing sphingomyelin synthase gene family. Detection of RV-infected cells by IFA. JAR cell clone #4 lacking SGMS1 were transiently express the indicated genes fused to mApple, followed by infection of either RVVAC (upper panels) or RVFI (lower panels) at 2 days post transfection. RV antigens were detected by IFA at 4 days post transfection, as shown in green. Parental JAR cells were shown as a positive control. A red fluorescent protein mCherry was used as a vector control. Blue represents the DAPI-stained nucleus; magnification 400×. Scale bar, 10 μm.