IVD of Chicken Egg Allergy

Background

Chicken eggs are a popular natural food rich in high-quality protein, essential amino acids, and trace elements. Current research shows that most allergens in eggs are found in egg whites. Ovalbumin is one of the primary proteins in egg whites and is a common allergen responsible for egg allergies. Ovomucoid (Gal d l), ovalbumin (Gal d 2), ovotransferrin (Gal d 3) and lysozyme (Gal d 4) are the main allergen proteins that can combine with human serum IgE to cause allergic reactions. The main clinical manifestations of egg allergy are skin symptoms, such as eczema-like rash, and skin erythema, accompanied by abdominal pain, vomiting, respiratory asthma, etc.

IVD of Chicken Egg Allergy

Main Steps of IVD for Chicken Egg Allergy

  • Detection of specific IgE in serum. Egg allergy can be diagnosed by detecting the level of specific IgE in the blood and combining it with routine blood tests.
  • Skin prick test. It is possible to diagnose allergies by pricking or injecting egg extract into the skin if the skin allergen prick result is positive and accompanied by relevant clinical symptoms.

Creative BioMart provides high-quality recombinant chicken egg allergen protein used for IVD, including ELISA, lateral flow assay, western blot, and other immunoassays.

Causes of Egg Allergy

  • Immune System Reaction: The immune system mistakenly identifies certain egg proteins as harmful, releasing histamines and other chemicals that cause allergic symptoms.
  • Genetics: A family history of allergies, particularly asthma or other food allergies, increases the risk.
  • Age: Egg allergy is most common in young children. As they grow older, their digestive systems mature, reducing the likelihood of maintaining the allergy.

Symptoms of Egg Allergy

  • Skin Reactions: Hives, eczema, or other skin-related symptoms.
  • Respiratory Issues: Nasal congestion, runny nose, sneezing, or other respiratory symptoms.
  • Gastrointestinal Symptoms: Cramps, nausea, vomiting, or diarrhea.
  • Anaphylaxis: A severe, potentially life-threatening reaction that requires immediate medical attention.

Sudden Onset of Egg Allergy

  • Changes in Immune System: Sometimes, an individual's immune system might change due to various factors such as infections, stress, or new exposures, which could trigger a new allergy.
  • Unknown Factors: Often, no clear reason is identified for the sudden onset of food allergies in adults.

Can You Eat Other Eggs?

  • Different Bird Eggs: Some people allergic to chicken eggs may react to eggs from other birds like duck, quail, or goose due to similar proteins. However, this varies individually. It is essential to consult with an allergist before trying other types of eggs.
  • Processed Forms: Some egg-allergic individuals can tolerate baked goods or products containing well-cooked eggs, where the proteins have been altered sufficiently to avoid reactions. However, this should be confirmed through allergy testing and under medical supervision.

Highlights of Our Products

  • High purity. No contamination from exogenous toxic substances and pathogenic microorganisms.
  • High specificity. Genetic engineering technology allows it to be produced without non-specific allergens.
  • Higher safety and repeatability.
  • Utilized for the advancement of diverse immunotherapies and vaccinations, in addition to the production and preparation of numerous diagnostic reagents.

Our Outstanding Advantages

  • Strong technical team, advanced scientific research equipment, and technology that can provide high-quality services.
  • Diverse IVD products to meet the different needs of customers and provide customers with comprehensive scientific research support.
  • Concentrate on service quality, provide customers with timely and accurate IVD-related services, and ensure that customers can get the best scientific research experience.

Case Study

Case 1: De Silva C, Dhanapala P, King S, Doran T, Tang M, Suphioglu C. Immunological Comparison of Native and Recombinant Hen's Egg Yolk Allergen, Chicken Serum Albumin (Gal d 5), Produced in Kluveromyces lactis. Nutrients. 2018 Jun 12;10(6):757. doi: 10.3390/nu10060757. PMID: 29895783; PMCID: PMC6024705.

Chicken serum albumin (CSA) is a hen's egg yolk allergen causing IgE-mediated allergy. The objective of this study was to produce a recombinant version of CSA and compare its IgE reactivity to natural CSA (nCSA). rCSA produced in this study is a potential candidate to be used in component-resolved diagnosis (CRD) of egg yolk allergy.

Fig2. Comparison of the binding activity of human sIgE against recombinant CSA and natural CSA. N denotes serum from healthy subjects and numbers denote different patients' sera sensitised to nCSA.

Case 2: Nagakura KI, Sato S, Shinahara W, et al. Effect of Maternal Egg Intake During the Early Neonatal Period and Risk of Infant Egg Allergy at 12 Months Among Breastfeeding Mothers: A Randomized Clinical Trial. JAMA Netw Open. 2023 Jul 3;6(7):e2322318. doi: 10.1001/jamanetworkopen.2023.22318. PMID: 37428506; PMCID: PMC10334231.

Egg introduction in infants at age 4 to 6 months is associated with a lower risk of immunoglobulin E-mediated egg allergy (EA). However, whether their risk of EA at age 12 months is affected by maternal intake of eggs at birth is unknown. This article is objective to determine the effect of maternal egg intake during the early neonatal period (0-5 days) on the development of EA in breastfed infants at age 12 months.

Fig3. Ovalbumin (OVA) and Ovomucoid (OVM) Levels in Breast Milk After Ingestion of a Whole Egg or During the Elimination of Eggs A, OVA levels in the maternal egg consumption (MEC) group. B, OVM levels in the MEC group. C, OVA levels in the maternal egg elimination (MEE) group. D, OVM levels in the MEE group. The midwife assisted and collected breast milk at a time when the mother was not feeding.