Lipid-based Protein Immobilization

The Lipid-based Protein immobilization technology (LiPi^™), developed in collaboration with pharmaceutical and academic groups, enables immobilization of native membrane proteins in the form of proteoliposomes, generated directly from cells or tissue.

The LiPi™ technology is based on the use of proprietary surfaces and sample preparation protocols designed to retain the natural environment around the membrane proteins. The membrane proteins can be subjected to a variety of protocols in a highly controlled manner, to provide effective and precise preparation of samples for downstream analysis.

LiPi™ FlowCell is the first product based on the LiPi™ technology and is used in applications such as functional studies, absolute quantification, epitope identification and sequencing studies amongst others. Additional products are in development, where other formats will complement LiPi™ FlowCell and allow straightforward expansion of experimental protocols and applications.

Applications:

• Ligand binding studies.
• Antigen epitope identification.
• Quantitative proteomics.
• Protein sequencing with multi-step digestion.
• Easy and efficient membrane protein profiling.

Key Features:

• Developed protocols for membrane preparations.
• Extensive knowledge in membrane proteomics.
• Unique in-house developed LiPi™ technology for sample preparation prior MS analysis.
• Optimized protocols for a wide variety of applications.
• State-of-the-art MS facility.
• Experience in analyzing complex membrane protein samples.
• Highly skilled bioinformatics group specialized in interpreting complex membrane protein data.
• In-house developed protein annotation database.