Fc Fusion Protein Production

Creative BioMart has become leading manufacturer of protein products for life science research. One of our specialties is to develop unique immunoglobulin based chimeric fusion proteins utilizing cellular and molecular biology techniques.

What is Fc Fusion Protein?

Due to the nature of the antibody IgG-Fc, Fc fusion proteins play significant role in dimerization, in vivo half-life, receptor activation and ligand affinity. Considering many receptors are only bio-functional as dimeric form, a Fc fusion receptor protein can mimic the activated form and enhance its affinity to ligand. Besides, Fc domain also simplifies its detection (by western blot or ELISA) & purification (by protein A or protein G affinity chromatography) steps. Mammalian cell-specific glycosylation from the Fc domain of the protein also makes itself suitable for in vivo studies. If desired, the Fc domain can be cleaved by treatment with protease.

Application of Fc Fusion Protein in Biopharmaceutical Industry

In the biopharmaceutical industry, as novel biological entities with potential for therapeutic activities, Fc-fusion proteins have become a class of biologic medicines for human disease. In some cases, these fusions are used as standalone therapeutic modalities; in others, they have provided alternatives when therapeutic monoclonal antibodies (mAbs) or other medications have failed to provide successful therapeutic results.

Why Choose Us for Fc Fusion Protein Production?

• Possess 20+ protein expression systems
• Years of industry experience and professional protein production team
• Ability to design individualized protein production protocols
• One-stop service from gene synthesis to protein quality testing
• Competitive prices in the industry to ensure cost-effectiveness
• Provide comprehensive experimental reports and analysis reports

Service Content of Fc Fusion Protein Production

1

Gene Synthesis Plus Codon Optimization

• Leading codon optimization system
• Analysis and optimization of rare codons, codon bias and other factors

Timeline: 1-2 weeks

2

Vector Construction

• Clone target gene into expression vector
• Plasmid sequencing
• Plasmid preparation

Timeline: 1 week

3

Transformation

• Transfect competent cells with transfer vector
• Various transformation methods

Timeline: 1 week

4

Protein Expression and Purification

• Expression condition optimization
• Protein expression evaluation
• Various protein purification methods

Timeline: 2-3 weeks

5

Optional Services

• Secondary purification
• Endotoxin removal
• Protein labeling
• Activity assay
• Protein analysis
• Other more

Timeline: 1-2 weeks

6

Quality Control

• SDS-PAGE
• Western blot
• Mass spectrometry

Timeline: <1 week

Deliverables

• Purified protein (supplied in liquid or lyophilized form)
• Gene sequencing report
• QC report
• SDS-PAGE/ HPLC/ SEC
• Activity data if applicable

The Fc options include but are not limited to:

• Human IgG1-Fc, IgG2-Fc, IgG3-Fc, IgG4-Fc
• Mouse IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc
• Rat IgG1-Fc, IgG2a-Fc, IgG2b-Fc, IgG3-Fc