RFK

  • Official Full Name

    riboflavin kinase
  • Overview

    Riboflavin kinase (RFK; EC 2.7.1.26) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin;B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis;(Karthikeyan et al., 2003 (PubMed 12623014)).
  • Synonyms

    RFK;riboflavin kinase;FLJ11149;RIFK;flavokinase;0610038L10Rik;ATP:riboflavin 5-phosphotransferase;RP11-422N19.2

Recombinant Proteins

  • Human
  • Mouse
  • Chicken
  • E.coli
  • Mammalian Cells
  • Human
  • HEK293
  • GST
  • His
  • Non
  • Myc&DDK
  • His&Fc&Avi
Cat.# Product name Source (Host) Species Tag Protein Length Price
RFK-2260H Recombinant Human RFK, GST-tagged E.coli Human GST 1-162aa
RFK-107H Recombinant Human Riboflavin Kinase E.coli Human His 1-162 a.a.
RFK-14102M Recombinant Mouse RFK Protein Mammalian Cells Mouse His
RFK-30014TH Recombinant Human RFK, His-tagged E.coli Human His Full L.
RFK-3243C Recombinant Chicken RFK Mammalian Cells Chicken His
RFK-8617H Recombinant Human RFK protein, His-tagged E.coli Human His Met1-His155
RFK-897H Recombinant Human Riboflavin Kinase, His-tagged Human Human His 1-162 a.a.
RFK-2407HCL Recombinant Human RFK 293 Cell Lysate HEK293 Human Non
RFK-1608H Recombinant Human RFK Protein, Myc/DDK-tagged, C13 and N15-labeled HEK293 Human Myc&DDK
Rfk-5478M Recombinant Mouse Rfk Protein, Myc/DDK-tagged HEK293 Mouse Myc&DDK
RFK-7541M Recombinant Mouse RFK Protein, His (Fc)-Avi-tagged HEK293 Mouse His&Fc&Avi
RFK-7541M-B Recombinant Mouse RFK Protein Pre-coupled Magnetic Beads HEK293 Mouse
RFK-7855H Recombinant Human RFK protein, His-tagged E.coli Human His 177aa(including fusion tag)

    Background

    RFK-5.jpg

    Fig1. A schematic model illustrating the functional coupling of TNFR1 to NADPH oxidase by RFK.

    What is RFK protein?

    RFK (Riboflavin Kinase) gene is a protein coding gene which situated on the long arm of chromosome 9 at locus 9q21. The gene encodes protein called Riboflavin kinase (RFK) consisted of 155 amino acids. This protein is approximately 17.6 kDa, also known as flavokinase, belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor.

    What is the function of RFK protein?

    RFK is an enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence it is a rate-limiting enzyme in the synthesis of FAD. RFK is essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, RFK physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase.

    RFK Related Diseases

    Dysfunctional RFK results in riboflavin deficiency, which further affects the synthesis of two coenzymes, FMN and FAD, leading to neurodegenerative diseases like Alzheimer's Disease.

    Bioapplications of RFK

    RFK may play a role in the protection of the nervous system and has potential implications for the treatment or prevention of diseases associated with neuronal ischemia, such as ischemic brain injury. RFK is a key enzyme in riboflavin metabolism and is specifically expressed in microglia which has important implications for understanding the physiological and pathological processes of the nervous system.

    Case Study

    Case Study 1: Mengran Zhang, 2023

    RFK-2.jpg

    Fig1. RFK is mainly located in microglia and its expression is increased in the inflammation-based cognitive impairment model.

    Case Study 2: Mengran Zhang, 2023

    RFK-3.jpg

    Fig2. Representative blots and quantification showing RFK expression levels in Control and LPS-treated BV2 cells n = 3 per group.

    Quality Guarantee

    High Purity

    Involved Pathway

    RFK involved in several pathways and played different roles in them. We selected most pathways RFK participated on our site, such as Riboflavin metabolism,Metabolic pathways, which may be useful for your reference. Also, other proteins which involved in the same pathway with RFK were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

    Pathway Name Pathway Related Protein
    Riboflavin metabolism BLVRB,P22,TYR,FLAD1
    Metabolic pathways GATC,NDUFB4,MGAT4A,ackA,SPTLC1,DCTPP1,CRLS1,MTHFD1L,PLA2G12A,SGMS1

    RFK-4.jpg

    Fig1. KMT2B motivates MIRI-induced cellular injury and ferroptosis by inducing RFK transcription and mediating the TNF-α/NOX2 axis.

    Protein Function

    RFK has several biochemical functions, for example, ATP binding,metal ion binding,riboflavin kinase activity. Some of the functions are cooperated with other proteins, some of the functions could acted by RFK itself. We selected most functions RFK had, and list some proteins which have the same functions with RFK. You can find most of the proteins on our site.

    Function Related Protein
    metal ion binding HIVEP2A,CNOT7,CYP19A1B,F13A1,LONRF1L,THOP1,GALNT10,MTMR4,MUL1B,TESK2
    ATP binding SMOK2B,RAD51D,AKT2,SGK196,MOS,PAPSS2B,MYO7AA,ABCA8,KIF22,SIK2A

    Interacting Protein

    RFK has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with RFK here. Most of them are supplied by our site. Hope this information will be useful for your research of RFK.

    RAB1A;kipA;DNAJB9

    Resources

    References

    • Park, KJ; Lee, CH; et al. Death Receptors 4 and 5 Activate Nox1 NADPH Oxidase through Riboflavin Kinase to Induce Reactive Oxygen Species-mediated Apoptotic Cell Death. JOURNAL OF BIOLOGICAL CHEMISTRY 287:3313-3325(2012).
    • Lin, J; Diamanduros, A; et al. Specific electron transport chain abnormalities in amyotrophic lateral sclerosis. JOURNAL OF NEUROLOGY 256:774-782(2009).
    • Miao, WM; Seng, WL; et al. Thrombospondin-1 type 1 repeat recombinant proteins inhibit tumor growth through transforming growth factor-beta-dependent and -independent mechanisms. CANCER RESEARCH 61:7830-7839(2001).

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