MBD1
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Official Full Name
methyl-CpG binding domain protein 1 -
Overview
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD).The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) colocalize with foci of heavily methylated satellite DNA and mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA either in vivo. MBD1, MBD2, MBD3, and MBD4 are expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 has homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract. -
Synonyms
MBD1;methyl-CpG binding domain protein 1;methyl-CpG-binding domain protein 1;CXXC3;PCM1;CXXC-type zinc finger protein 3;protein containing methyl-CpG-binding domain 1;methyl-CpG binding domain protein 1 isoform PCM1;the regulator of fibroblast gro
| Cat.# | Product name | Source (Host) | Species | Tag | Protein Length | Price |
|---|---|---|---|---|---|---|
| MBD1-1064HCL | Recombinant Human MBD1 cell lysate | Human | Non |
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| MBD1-2425H | Recombinant Human MBD1 Protein, His-tagged | E.coli | Human | His | Met1-Leu216 |
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Involved Pathway
MBD1 involved in several pathways and played different roles in them. We selected most pathways MBD1 participated on our site, such as SIDS Susceptibility Pathways, which may be useful for your reference. Also, other proteins which involved in the same pathway with MBD1 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
|---|---|
| SIDS Susceptibility Pathways | POU3F2,TPH2,RUNX3,PHOX2A,DLX2,TSPYL1,MAP2,MAZ,TPPP,ECE1 |
Protein Function
MBD1 has several biochemical functions, for example, DNA binding,chromatin binding,double-stranded methylated DNA binding. Some of the functions are cooperated with other proteins, some of the functions could acted by MBD1 itself. We selected most functions MBD1 had, and list some proteins which have the same functions with MBD1. You can find most of the proteins on our site.
| Function | Related Protein |
|---|---|
| double-stranded methylated DNA binding | MECP2,H2AFY |
| protein binding | POR,SPRR3,TMEM182,CAND2,RAD18,MYCBP,SF3A1,SFRP1,NELL2,BBS10 |
| zinc ion binding | DEF8,GRIN2A,PHF10,VDRB,Adam6,ITGB1BP2,DPP3,ZNF253,NR2F1B,Car2 |
| DNA binding | NFE2L2A,SEBOX,HMGB1B,ARID5B,TFDP1,HOXD10A,TOX3,ZNF791,FOXA2,ZNF576 |
| chromatin binding | ARX,DNMT3B,TCF3,LRWD1,NFIA,KLHDC3,ZFP932,MPO,SP140,TCF7L2 |
| methyl-CpG binding | UHRF1,MBD3B,MBD3A,ZBTB4,MBD3L1,ZBTB33,MBD1B,ZNF295,LRWD1,MBD3 |
Interacting Protein
MBD1 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with MBD1 here. Most of them are supplied by our site. Hope this information will be useful for your research of MBD1.
CBX5;HDAC3;PIAS1;CHAF1A;SETDB1;PIAS3
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References
- Xu, ZN; Yin, HS; et al. Electrochemical immunosensor for DNA methyltransferase activity assay based on methyl CpG-binding protein and dual gold nanoparticle conjugate-based signal amplification. SENSORS AND ACTUATORS B-CHEMICAL 192:143-149(2014).
- Yin, HS; Sun, B; et al. A new strategy for methylated DNA detection based on photoelectrochemical immunosensor using Bi2S3 nanorods, methyl bonding domain protein and anti-his tag antibody. BIOSENSORS & BIOELECTRONICS 51:103-108(2014).
