GAS2L3

What is GAS2L3 Protein?

GAS2L3 is an intriguing protein that's becoming a hot topic for its role in cell behavior. It's a member of the GAS2 family and acts as a bridge between actin and microtubule networks, which are crucial for cell shape and function. Think of it as a connector that helps with cell division, growth, and movement. This protein is found across many species, showing up in various human tissues and underscoring its importance. What’s unique about GAS2L3 is how it links with both actin and microtubules through distinct domains, unlike its relatives that mainly stick to microtubules. This feature gives it a special role in cell activities. Studying GAS2L3 opens up new understanding of its part in cellular workings and what it might mean for health, making it a focus of interest in cell biology research.

What is the Function of GAS2L3 Protein?

GAS2L3 plays a crucial role in keeping cells in shape and on the move. It connects the actin and microtubule networks inside cells, helping maintain their structure. This link is essential for processes like cell division, movement, and growth. What makes GAS2L3 stand out is its ability to bind to both actin and microtubules, unlike other proteins that usually focus on just one. This dual-action helps ensure proper communication between these two networks, which is vital for maintaining cell integrity. Researchers are looking into how GAS2L3's function impacts cell division and could relate to diseases, making it a hot topic for understanding how cells work and how they might go wrong in various conditions.

GAS2L3 Related Signaling Pathway

The GAS2L3 pathway is crucial in helping cells decide when to grow, divide, or take a break, ensuring everything stays in balance. Understanding this pathway is a bit like learning the rules of a complex board game, where each move impacts the next. Keywords like signaling pathways and cellular communication fit perfectly into this storyline. When things go awry, it can lead to disorders like cancer, but by studying pathways like GAS2L3, scientists can unlock new ways to treat these issues, keeping everything in sync and ensuring a healthy cellular environment.

GAS2L3 Related Diseases

GAS2L3-related diseases occur when disruptions in this crucial signaling pathway lead to cellular miscommunication, akin to crossed wires causing chaotic signals. This pathway plays a vital role in regulating the cell cycle, and any disruptions can pave the way for conditions such as cancer and developmental disorders. By focusing on keywords like cellular miscommunication, cancer research, and cell cycle disorders, scientists aim to unravel the mysteries within the GAS2L3 pathway. Understanding these disruptions not only highlights where things go wrong, but also opens up new therapeutic avenues to rectify these cellular imbalances and combat the associated diseases effectively.

Bioapplications of GAS2L3

GAS2L3’s role in cell division and maintaining structural integrity makes it a promising candidate for various bioapplications in medicine and biotechnology. Researchers are actively investigating its potential in cancer therapy and tissue regeneration. By better understanding how GAS2L3 functions, scientists aim to harness its capabilities to develop innovative treatments for diseases linked to cell cycle disruptions. This could include techniques for targeted cancer treatment or advanced methods in tissue engineering, ultimately aiming for more effective and precise medical interventions.

Case Study 1: Wolter P. et al. J Cell Sci. 2012

The mammalian DREAM complex plays a key role in controlling cell cycle-related genes. In digging into this, researchers found that GAS2L3, part of the GAS2 protein family, is a DREAM target and important for cell division. GAS2L3 is found at the spindle midzone and midbody during key cell division stages. It helps bundle microtubules and F-actin. When GAS2L3 levels drop due to RNAi, cells show messed-up chromosome segregation and weirdly shaped nuclei. Long-term depletion even causes chromosome loss. Videos of cell division in these cells showed erratic chromatin and spindle movement. All this points to GAS2L3 being essential for smooth cell division, linking back to DREAM’s role in the process.

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  Fig1. Expression of GAS2L3 in HeLa cells stably expressing a tetracycline-inducible and HA- and streptavidin-binding-petide-tagged-GAS2L3 was analyzed by immunoblotting.
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  Fig2. GAS2L3 crosslinks F-actin and microtubules.

Case Study 2: Stroud MJ. et al. J Biol Chem. 2011

The microtubule (MT) and actin cytoskeletons are crucial for cell structure and activities like division, growth, and movement. Proteins that link MTs and actin are vital for these processes. Researchers discovered G2L3, a new member of the GAS2 protein family, which plays a big role in this cross-talk. G2L3 is conserved across species and found in human tissues. It binds to filamentous actin and MTs through its calponin homology domain and its C terminus. Unlike similar proteins, the GAS2-related domain in G2L3 adjusts how it binds to both actin and MTs, rather than just binding MTs. Plus, they found that tubulin acetylation helps G2L3 target MTs, offering new clues about its function.

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  Fig3. Actin binding assays were performed using recombinant versions of G2L3-CH (CH) and G2L3-C-term (C-term).
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  Fig4. MT-spin down assays were performed using G2L3-CH (CH) and G2L3-GAR (GAR) as well as G2L3-C-term.

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  Fig1. Model of G2L-mediated crosstalk. (Matthew J Stroud, 2014)

GAS2L3 has several biochemical functions, for example, actin binding,microtubule binding,protein binding. Some of the functions are cooperated with other proteins, some of the functions could acted by GAS2L3 itself. We selected most functions GAS2L3 had, and list some proteins which have the same functions with GAS2L3. You can find most of the proteins on our site.

Function	Related Protein
microtubule binding	MACF1,NUMA1,RACGAP1,KIFC2,KIF25,SYBU,NEFH,CCDC88A,KIF3A,KIF20A
actin binding	DIXDC1,EPS8L2,SNTA1,MYO9B,ABLIM2,MYH7,FMNL2,WASF3,TPM4,FSCN1
protein binding	MALL,LDHD,RIT1,HECTD3,ISCU,IGF2,CTAGE5,ASL,MAP3K3,RAB5C

GAS2L3 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with GAS2L3 here. Most of them are supplied by our site. Hope this information will be useful for your research of GAS2L3.

AURKB;BIRC5;CDCA8;KIF20A;SPAST