OmpA
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Official Full Name
major outer membrane protein A -
Overview
The principal surface component of C. trachomatis, the major outer-membrane protein (MOMP), is a prominent determinant in serotyping and is presumed to be important in the development of protective immunity. Monoclonal antibodies (mAbs) to surface-exposed -
Synonyms
ompA;Bacterial outer membrane protein;outer membrane protein A;MOMP;omp1;omp1F;Omp1L1;ompIL2;Chlamydia trachomatis;C. trachomatis;ompIL3;ompIL4;ompIL5;ompIL6;ompIL7;ompIL8
Recombinant Proteins
- Bacterial
- Chlamydia trachomatis serovar L2
- Zebrafish
- Chlamydia Pneumonia
- Klebsiella pneumoniae
- Chlamydia trachomatis
- Salmonella Typhi
- E.coli
- Haemophilus Influenzae
- Chlamydia pecorum
- Chlamydia psittaci
- Rickettsia conorii
- Escherichia fergusonii
- Serratia odorifera
- Shigella dysenteriae
- Escherichia hermannii
- Citrobacter freundii
- E.coli
- E. coli
- Mammalian Cell
- In vitro E. coli expression system
- Insect cells
- E.coli expression system
- Non
- N-His-SUMO
- His
- His&Myc
- His&SUMO
- His&Myc&SUMO
- N-His&C-Myc
Background
What is OMPA protein?
The OMPA protein, also known as Outer Membrane Protein A, is a protein found in the outer membrane of Gram-negative bacteria. It is a major constituent of the outer membrane and plays various roles in bacterial physiology and pathogenesis. OMPA is involved in maintaining the structural integrity of the outer membrane, serving as a barrier to protect the bacteria from environmental stresses such as antibiotics and host immune responses. Additionally, OMPA is responsible for regulating the permeability of small molecules across the outer membrane.
What is the function of OMPA protein?
The main function of OMPA protein is to maintain the structural integrity of the bacterial outer membrane by forming trimeric porin channels. These channels allow the passive diffusion of small molecules, such as nutrients and ions, across the outer membrane. OMPA porins are known to facilitate the uptake of sugars, amino acids, and other essential compounds by the bacteria.
Additionally, OMPA protein is involved in the protection of bacteria from external stresses, including environmental toxins and antibiotics. It acts as a barrier and reduces the permeability of the outer membrane, thereby providing protection against harmful substances.
OMPA related signaling pathway
Ion Transport: OMPA can facilitate the transport of small ions, such as K+, Na+, and H+, across the bacterial outer membrane. This is important for maintaining osmotic balance and pH regulation in the cell.
Nutrient Uptake: OMPA can also play a role in the uptake of nutrients into the bacterial cell. It can form channels that allow the passage of small molecules, such as sugars and amino acids, into the cell.
Antibiotic Resistance: OMPA has been implicated in antibiotic resistance in some bacterial species. It can act as a barrier, preventing the entry of antibiotics into the cell or actively pumping them out through efflux pumps.
Virulence: In certain pathogens, OMPA has been associated with virulence and pathogenicity. It can contribute to the ability of the bacteria to evade the host immune system and establish infection.
OMPA Related Diseases
- Bacterial infections: OmpA can play a role in bacterial virulence and colonization by facilitating adhesion to host cells and evasion of the immune system. It is implicated in infections caused by bacteria such as Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, and Salmonella enterica.
- Inflammatory bowel disease (IBD): OmpA has been detected in the outer membrane of certain gut bacteria and has been associated with the development and progression of IBD, including Crohn's disease and ulcerative colitis.
- Vaccine development: OMPA is a major immunogenic protein of several bacterial pathogens, making it a potential antigen for vaccine development. It can stimulate an immune response and elicit the production of specific antibodies that can target these pathogens.
- Diagnostic tool: OMPA can be used as a diagnostic tool for detecting bacterial infections. Antibodies against OMPA can be used in immunoassays or rapid diagnostic tests to detect the presence of specific bacterial pathogens in patient samples.
Biomedical Application of OMPA Protein
- Vaccine development: OMPA is a major immunogenic protein of several bacterial pathogens, making it a potential antigen for vaccine development. It can stimulate an immune response and elicit the production of specific antibodies that can target these pathogens.
- Antibiotic targets: OMPA plays a crucial role in the permeability barrier of bacterial outer membranes. Therefore, it can be targeted by antibiotics to disrupt the integrity of the bacterial outer membrane, leading to the enhanced effectiveness of antibiotics against drug-resistant strains.
- Diagnostic tool: OMPA can be used as a diagnostic tool for detecting bacterial infections. Antibodies against OMPA can be used in immunoassays or rapid diagnostic tests to detect the presence of specific bacterial pathogens in patient samples.
Case Study
(Nathan J. Alves, 2015)
Fig2. A) SDS-PAGE and B) Western blot of the purified OMV UC pellets of all of the various constructs demonstrating relative abundance of OmpA-ST, native OmpA, PTE-SC, and the OmpA-ST/PTE-SC fusion. A His-tag was included in the mutant forms of OmpA and PTE to facilitate visualization via an anti6xHis antibody. PTE is known to dimerize, and this is evident on the blot by the presence of larger molecular weight His-tagged species.
(Timothy Robson, 2018)
Fig3. Characterization of AuNPs (preconjugated with wild type and cysOmpATM) before and after overnight thioAlkylPEG filler incubation. 20 nm AuNPs at OD525nm = 10 were incubated with 8 μM of either protein for 30 min, followed by coassembly with filler by mixing the protein–AuNP conjugates with 8 μM filler and incubating overnight. (A) 0.5% agarose gel of wtOmpATM particles before (1) and after filler addition (2) and cysOmpATM particles before (3) and after (4) filler (F) addition. Corresponding LSPR absorbance peak barycentric mean wavelength Δλm (B) and change in hydrodynamic radius (ΔDHyd) measured by DLS (C) data (where ΔDHyd = 0 corresponds to bare particles) for the same protein–AuNP conjugate samples used in (A). Error bars represent the standard error of the mean based upon three measurements.
Quality Guarantee
High Purity
Fig1. SDS-PAGE (OMPA-1906E)
Involved Pathway
OmpA involved in several pathways and played different roles in them. We selected most pathways OmpA participated on our site, such as , which may be useful for your reference. Also, other proteins which involved in the same pathway with OmpA were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.
| Pathway Name | Pathway Related Protein |
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Protein Function
OmpA has several biochemical functions, for example, . Some of the functions are cooperated with other proteins, some of the functions could acted by OmpA itself. We selected most functions OmpA had, and list some proteins which have the same functions with OmpA. You can find most of the proteins on our site.
| Function | Related Protein |
|---|
Interacting Protein
OmpA has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with OmpA here. Most of them are supplied by our site. Hope this information will be useful for your research of OmpA.
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