Source : |
HEK293 |
Species : |
Human |
Tag : |
C-His-Avi |
Protein length : |
GIn19-Pro139 |
Conjugation/Label : |
Biotin |
Form : |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
Bio-activity : |
Immobilized Biotinylated Human CD47, His Tag at 2μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human SIRP alpha, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. |
Molecular Mass : |
The protein has a predicted MW of 16.63 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result. |
Endotoxin : |
Less than 1EU per μg by the LAL method. |
Purity : |
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Storage : |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
Reconstitution : |
Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |