Creative Biomart Blog

Mon, 2016/02/01

Lack of Certain Transcription Factors May Promote Blood Vessel Growth

Blood Vessel plays a very important role as its growth determines whether timely nutrition is provided during embryonic development. In adulthood, the growth of new vessels can help the process of repair and regeneration. When the growth of them are disturbed, cancer, diabetes and eye disease may occur. By now, scientists have found that the growth of blood vessels in the innermost cell layer can be controlled by metabolism. The results, however, can be used as the basis of new treatments for diseases. Vascular is generally referred as water pipe; those pipeline system supply oxygen and nutr

Fri, 2016/01/15

Noteworthy Technology in 2016: Protein Labeling in Cells

Nature Method has recently sorted out the most noteworthy technologies in 2016, including protein labeling in cells, unraveling nuclear architecture, protein structure though time, precision optogenetics, highly multiplexed imaging, deep learning, subcellular maps and integrated single-cell profiles. Florescent dyes are relatively small, having good photophysical properties and spectral span. Those features make fluorescent dyes attractive, and they are expected to replace fluorescent protein for protein labeling. Researchers are actively developing tools to label protein by dyes in living c

Thu, 2015/12/10

Pigeons that Can Distinguish Cancer Image

In nature, numerous animals share characteristics with humans, such as pigeons, its ability to find ways still remains for human use. However, a recent study makes people aware it’s more than that. Richard Levenson, pathologists from the United States together with Edward Wasserman, psychologist from Edward Wasserman, trained pigeons to identify cancer. During training, the researchers first demonstrated the microscope image of breast tissue to pigeon. Then, the researchers would reward them if they can peck correctly the corresponding colored buttons of healthy or cancerous tissues. Each

Wed, 2015/11/25

Principle and Protocol of Enzyme-linked Immunosorbent Assay (ELISA)

The development of ELISA is based on the immobilization and enzyme labeling of antigen or antibody. The immunological activity of antigen or antibody remains after the immobilization. And enzyme-labeled antigen or antibody remains both their immunological activity and enzymatic activity. Materials: Antigen Serum Reagent and kit: Carbonate coating buffer PBST BSA Equipment and Supplies: 96-Well ELISA plates 4℃refrigerator Incubator Spectrophotometer Procedure: (1) Coating antigen 1. Dilute the antigen into 10-20μg/ml with 50 mM carbonate coating buffer (pH9.6) ; add 100μl per well

Sun, 2015/11/22

Principle and Protocol of Chromatin Immunoprecipitation (ChIP)

Introduction: 1. Applications of ChIP: (1) Histone modifying enzyme antibody as "biomarkers"; (2) Analysis of transcriptional regulation; (3) Drug development research; (4) Analysis of DNA damage and apoptosis. 2. Details: The eukaryotic genomic DNA exists in the form of chromatin. Therefore, the study of interactions between protein and DNA in chromatin environment is the basic way to clarify the mechanism of gene expression in eukaryotes. Chromatin immunoprecipitation (ChIP) is currently the only method to study DNA and protein interactions in vivo. Its basic principle is firstly to

Tue, 2015/11/17

Principle and Protocol of Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Principle The concentration of polyacrylamide gels can be prepared as required in two electrophoresis systems —called "continuous system" and "discontinuous system". The biggest feature of “discontinuous system” lies in its greatly improved sample separation resolution. Main features of this electrophoresis are: (1) Use of two gel systems with different concentrations; (2) Solution composition and pH are different for the preparation of the two gel and are also different from electrophoresis buffer composition and pH in electrophoresis tank. In the experiment, electrophoresis gel is divided i

Fri, 2015/10/30

Month-end Check: Biotech study topics in Nature-October

Gene-editing record smashed in pigs For a long time, scientists are working to create a steady supply of human organs for transplantation by growing them in pigs. But concerns about rejection by the human immune system and infection by viruses embedded in the pig genome have stymied research. In a current research, by modifying more than 60 genes in pig embryos-ten times than have been edited in other animal. Researchers believe they might have produced a suitable non-human organ supplier. Scientists hope to attract millions to 'DNA.LAND' Scientists in the field of genetics have launched a p

Wed, 2015/10/28

Sensing Proteins Prevents Obesity and Diabetes Found

Recently, researchers at the University of Melbourne determined an internal “sensor” which can help fight by indicating cells to burn their fat. This finding might play an important role in the treatment for obesity and metabolic diseases (including type 2 diabetes). In this study, the researchers found that when the increased dietary energy (food) intake triggers cells to become “unstable”, a protein called NLRP1 is opened. Activate this protein can trigger a series of events, indicating cells to use their energy or fat storage to prevent fat accumulation. Conducted by Dr. Seth Masters an