Description :
Pectinase is an enzyme that breaks down pectin, a polysaccharide found in plant cell walls. Commonly referred to as pectic enzymes, they include pectolyase, pectozyme and polygalacturonase. One of the most studied and widely used commercial pectinases is polygalacturonase. It is useful because pectin is the jelly-like matrix which helps cement plant cells together and in which other cell wall components, such as cellulose fibrils, are embedded. Therefore pectinase enzymes are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juice from fruit, including apples and sapota. Pectinases have also been used in wine production since the 1960s. The assay is initiated with the enzymatic hydrolysis of the pectin by pectinase. The enzyme catalysed reaction products react with DNS, and can be measured at a colorimetric readout at 540 nm.
Storage :
Shipped and store at 4 degree C for 6 months.
Kit Components :
96-Well Microplate: 1 plate
Assay Buffer: 30 ml x 4, 4 °C
Diluent: 10 ml x 1, 4 °C
Substrate: Powder x 1, 4 °C
Dye Reagent: 10 ml x 1, 4 °C, keep in dark
Standard: Powder x 1, 4 °C
Plate Adhesive Strips: 3 Strips
Technical Manual: 1 Manual
Note:
Substrate: add 8 ml Diluent to dissolve before use.
Standard: add 1 ml Diluent to dissolve before use, the concentration will be 2 mg/ml.
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