| Cat# | Product Name | Price |
|---|---|---|
| Kit-0417 | HDAC3/NCOR1 fluorometric drug discovery Kit | Inquiry |
| Kit-0431 | HDAC3 Activity Assay Kit | Inquiry |
| Kit-0432 | HDAC3 Inhibitor Screening Kit | Inquiry |
| Kit-2254 | HDAC3 Activity Fluorometric Assay Kit | Inquiry |
HDAC3 is a histone deacetylase belonging to the histone modifying enzyme family. It is the abbreviation of histone deacetylase 3. Its main function is to affect the interaction between DNA and histone by removing the acetylation modification on histone, so as to regulator gene transcription process. The biological functions of HDAC3 have various forms, mainly involving regulatory roles in smooth muscle development, metabolism, and cell proliferation and differentiation research.
HDAC3 is a highly conserved histone modifying enzyme widely present in the nucleus of mammalian cells, mainly mediating the deacetylation modification of histones. Histones are the main proteins in the nucleus, responsible for the encapsulation of DNA and maintaining gene stability. The absence or abnormal expression of HDAC3 can lead to dysregulation of histone modifications on DNA, leading to abnormal gene expression and uncontrolled cell proliferation.
For the determination of HDAC3 activity, fluorescent or radioactive derivative labeled peptide is usually used as the substrate, which generates fluorescent or radioactive molecules through hydrolysis to determine the activity of HDAC3. Among them, the forms and substrate concentrations of various peptide peptides may vary, which may affect the detection results and require appropriate selection. In addition, due to the dependence of HDAC3 on other cofactors such as Sin3 and NURD, special attention needs to be paid to and appropriate enzyme reaction systems need to be determined during the experimental process. Ultimately, modern experimental instruments such as fluorescence meters or radiometers can be used for measurement.
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