HDAC3 Activity Fluorometric Assay Kit

Cat.No. :

Kit-2254

Product Overview :

Histone deacetylases (HDACs) represent a large family of enzymes identified as key regulators of nucleosomal histone acetylation, a major event that controls eukaryotic gene transcription and are classified into three groups. HDACs are transcriptional repressors with crucial roles in mammalian development. They are believed to be involved in important biological activities, such as cell differentiation, proliferation, apoptosis, and senescence. In HDAC-3 activity Kit, HDAC-3 and Developer will deacetylate and cleave the substrate [R-H-K-K(Ac)-AFC] to release the quenched fluorescent group (AFC), which can be detected at Em/Ex = 380/500 nm. The kit provides a rapid, simple, sensitive, and reliable test, which is also suitable for high throughput measurement of HDAC-3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC3 samples.

Applications :

Suitable for high throughput measurement of HDAC3 activity in purified, immunoprecipitated and recombinant or genetically modified HDAC-8 samples.

Storage :

-20°C

Shipping :

Gel Pack

Size :

100 assays

Kit Components :

HDAC3 Assay Buffer; HDAC3 Substrate; HDAC3 Positive Control; AFC Standard (1 mM); Developer; Trichostatin A

Detection method :

Fluorescence (Em/Ex = 380/500 nm)

Features & Benefits :

Simple procedure; takes ~ 60 min;
Fast and convenient;
Kit contains the necessary reagents for accurate measurement of HDAC3 activity