Active Recombinant Human CDC7/DBF4 Protein, GST-tagged

• Cat.No.: CDC7-22H
• Product Overview: Recombinant Human CDC7/DBF4 fused with GST tag at N-terminal was expressed in Insect cells.

Specification

• Species: Human
• Source: Insect Cells
• Tag: GST
• Description: Recombinant full-length human CDC7 and DBF4 proteins were co-expressed by baculovirus in Sf9 insect cells using an N-terminal GST tags. CDC7 is a cell division cycle 7 homolog protein that is critical for the G1/S transition and is also essential for initiation of DNA replication as cell division occurs. CDC7 is expressed in many normal tissues, but the overexpression of CDC7 may be associated with neoplastic transformation for some tumors and transformed cell lines. CDC7/DBF4 kinase promotes S phase by alleviating an inhibitory activity in Mcm4 that evolved to integrate several protein kinase.
• Form: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Bio-activity: 8.5 nmol/min/mg
• Molecular Mass: CDC7 ~94kDa and DBF4 ~125kDa
• Purity: > 85%
• Applications: Kinase Assay, Western Blot
• Stability: 1 year at -70 centigrade from the date of shipment.
• Storage: Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Concentration: 0.1 µg/µl

Gene Information

• Gene Name: CDC7 cell division cycle 7 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: CDC7
• Synonyms: CDC7; cell division cycle 7 homolog (S. cerevisiae); CDC7 (cell division cycle 7, S. cerevisiae, homolog) like 1 , CDC7 cell division cycle 7 (S. cerevisiae) , CDC7L1, cell division cycle 7 (S. cerevisiae); cell division cycle 7-related protein kinase; HsCdc7; Hsk1; huCdc7; CDC7-related kinase; cell division cycle 7-like protein 1; CDC7 (cell division cycle 7, S. cerevisiae, homolog)-like 1; CDC7L1; HsCDC7; huCDC7; MGC117361; MGC126237; MGC126238
• Gene ID: 8317
• mRNA Refseq: NM_001134419
• Protein Refseq: NP_001127891
• MIM: 603311
• UniProt ID: O00311