Liquid chromatography tandem mass spectrometry/mass spectrometry (LC-MS/MS) provides absolute measures of 5hmC content in genomic DNA. It’s highly accurate and sensitive and is regarded as the ‘gold standard’ for global DNA hydroxymethylation measurement. Creative BioMart provides customers with a global DNA 5hmC quantification service with solid expertise and state-of-the-art LC-MS/MS equipment.

What Is LC-MS/MS for Global DNA 5hmC Quantification?

In this assay, genomic DNA is enzymatically digested into normal and modified mononucleotides, which are then separated by high performance liquid chromatography (HPLC) and then detected by the mass spectrometer. Due to the efficient separation provided by HPLC and structure-based mass spectrometry, LC-MS/MS can perform specific and sensitive detection of various modifications of DNA. Recently developed methods based on changes in liquid chromatography technology, such as liquid chromatography electrospray ionization tandem mass spectrometry with multiple reaction monitoring (LC-ESI-MS/MS-MRM), have pushed the limits of epigenetic analysis and subsequently utilized to quantification of 5fC and 5caC in addition to 5mC and 5hmC.

Features of Global DNA 5hmC Quantification by LC-MS/MS

1. Independent of DNA quality
2. Extremely high sensitivity, specificity, and repeatability
3. Independent of sequence, thus can be utilized to analyze DNA from various species
4. The measurement results reflect the absolute level of global DNA hydroxymethylation and is not suitable for determining the precise location of the 5hmC modification

Our Advantages

1. Tolerance of multiple sample types: we accept a wide range of biological and clinical samples, including but not limited to, genomic DNA isolated from a variety of species, cultured cells, fresh/frozen tissues, formalin-fixed and paraffin-embedded (FFPE) samples.
2. One-stop service: customers only need to provide samples, and Creative BioMart will complete the full LC-MS/MS experiment and data analysis.
3. State-of-the-art technology platform: we have state-of-the-art LC-MS/MS equipment, and our technical team has accumulated years of expertise and experience in complicated mass spectrometry specifications.
4. Competitive pricing & Short turnaround time: Creative BioMart is committed to long-term partnerships, so services provided by us have competitive price and short turnaround time.

Workflow of Global DNA 5hmC Quantification by LC-MS/MS at Creative BioMart

Figure 1. Workflow of Global DNA 5hmC Quantification by LC-MS/MS at Creative BioMart

If you have any special requirements or questions in global DNA 5hmC quantification by LC-MS/MS, please don’t hesitate to contact us. We are looking forward to working together with your attractive projects.