| Cat.No.: | PE-1138 |
| Product Name: | Recombinant Human KDM1A, FLAG-tagged |
| Product Overview: | Recombinant Human KDM1A (accession number NP_055828) was expressed in Sf9 cells and contains an N-terminal FLAG tag with a molecular weight of 97.1 kDa. |
| Description: | LSD1 (Lysine-specific demethylase 1, also designated KDM1A) was identified as a subunit of different complexes (such as CTBP, CoREST, NuRD or BRAF35). LSD1 is involved in transcriptional repression of genes through the demethylation of Histone H3 monomethyl Lys4 and Histone H3 dimethyl Lys4, a methylation site associated with transcriptional activation. LSD1 is also involved in transcriptional activation through the demethylation of Histone H3 monomethyl Lys9 and Histone H3 dimethyl Lys9, a modification often associated with transcriptional repression. LSD1 is also able to demethylate Lys370me1 and Lys370me2 in the regulatory domain of the tumor suppressor p53. LSD1 is a flavin-dependent amine oxidase. |
| Applications: | Enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Species: | Human |
| Formulation: | 20 mM Tris-HCl pH 7.4, 150 mM NaCl and 20% glycerol. |
| Tag: | FLAG |
| Activity: | H3K4me2 and H3K4me1 demethylase. |
| Expression System: | Sf9 Insect Cells |
| Concentrations: | 0.77 mg/ml |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | KDM1A lysine (K)-specific demethylase 1A [ Homo sapiens ] |
| Gene ID NCBI: | 23028 |
| Synonyms: | KDM1A; lysine (K)-specific demethylase 1A; amine oxidase (flavin containing) domain 2 , AOF2, KDM1, lysine (K) specific demethylase 1; lysine-specific histone demethylase 1A; BHC110; KIAA0601; LSD1; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; lysine-specific histone demethylase 1; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-containing protein 2; AOF2; KDM1; |
| mRNA Refseq: | NM_001009999 |
| Protein Refseq: | NP_001009999 |
| MIM: | 609132 |
| UniProt ID: | O60341 |
| Chromosome Location: | 1p36.12 |
| Function: | MRF binding; androgen receptor binding; chromatin binding; demethylase activity; enzyme binding; flavin adenine dinucleotide binding; histone demethylase activity; histone demethylase activity (H3-K4 specific); histone demethylase activity (H3-K9 specific); histone demethylase activity (H3-dimethyl-K4 specific); ligand-dependent nuclear receptor transcription coactivator activity; oxidoreductase activity; p53 binding; protein binding; transcription factor binding; transcription regulatory region DNA binding; |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
| CL-0088 | Human KDM1B Knockout Cell Line 13bp deletion | Inquiry |
| CL-0089 | Human KDM2B Knockout Cell Line 14bp deletion | Inquiry |
| ◆ Antibodies | ||
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| KDM1 | KDM1A | KDM1B | KDM2 |
| KDM2A | KDM2B | KDM3A | KDM3B |
| KDM4 | KDM4A | KDM4B | KDM4C |
| KDM4D | KDM4E | KDM5 | KDM5A |
| KDM5B | KDM5C | KDM5D | KDM6A More > |
| KDM6B | KDM6C | KDM7 | KDM7A |
| KDM7B | KDM8 | ||
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