| Cat.No.: | PE-1083 |
| Product Name: | Recombinant Human DOT1L, GST-tagged |
| Product Overview: | Recombinant human DOT1 protein, catalytic domain (DOT1L, KMT4, accession Q8TEK3, amino acids 1-472), containing an N-terminal GST tag, expressed in E. coli |
| Description: | The protein encoded by this gene is a histone methyltransferase that methylates lysine-79 of histone H3. It is inactive against free core histones, but shows significant histone methyltransferase activity against nucleosomes. |
| Applications: | Recombinant DOT1L Catalytic Domain, human is useful for histone H3 methylation experiments, enzyme kinetics and inhibitor screening. Use of 3-5 µg DOT1L per reaction with HeLa or recombinant nucleosomes as a substrate is recommended. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 85 kDa |
| Purity: | 70% as measured by gel densitometry. |
| Species: | Human |
| Formulation: | Recombinant GST-DOT1L (1 µg/µl) 100 mM Tris pH 8.0, 5 mM glutathione and 25% glycerol. |
| Tag: | GST |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | DOT1L DOT1-like histone H3K79 methyltransferase [ Homo sapiens (human) ] |
| Gene ID NCBI: | 84444 |
| Synonyms: | DOT1L; DOT1; KMT4; DOT1-like histone H3K79 methyltransferase; histone-lysine N-methyltransferase, H3 lysine-79 specific; H3-K79-HMTase; DOT1-like protein; lysine N-methyltransferase 4; histone methyltransferase DOT1L; histone H3-K79 methyltransferase; DOT1-like, histone H3 methyltransferase; NP_115871.1; EC 2.1.1.43 |
| mRNA Refseq: | NM_032482 |
| Protein Refseq: | NP_115871 |
| MIM: | 607375 |
| Chromosome Location: | 19p13.3 |
| Function: | DNA binding; histone-lysine N-methyltransferase activity; protein binding |
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