| Cat.No.: | PE-0925 |
| Product Name: | Recombinant Human Cyclin B1, His-tagged |
| Product Overview: | Recombinant human CCNB1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques. |
| Description: | CCNB1 is a regulatory protein involved in mitosis. This protein complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. |
| Applications: | SDS-PAGE |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 50.9 kDa (457aa) |
| Purity: | >80% by SDS - PAGE |
| Species: | Human |
| Formulation: | Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol. |
| Tag: | His |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMALRVT RNSKINAENK AKINMAGAKR VPTAPAATSK PGLRPRTALG DIGNKVSEQL QAKMPMKKEA KPSATGKVID KKLPKPLEKV PMLVPVPVSE PVPEPEPEPE PEPVKEEKLS PEPILVDTAS PSPMETSGCA PAEEDLCQAF SDVILAVNDV DAEDGADPNL CSEYVKDIYA YLRQLEEEQA VRPKYLLGRE VTGNMRAILI DWLVQVQMKF RLLQETMYMT VSIIDRFMQN NCVPKKMLQL VGVTAMFIAS KYEEMYPPEI GDFAFVTDNT YTKHQIRQME MKILRALNFG LGRPLPLHFL RRASKIGEVD VEQHTLAKYL MELTMLDYDM VHFPPSQIAA GAFCLALKIL DNGEWTPTLQ HYLSYTEESL LPVMQHLAKN VVMVNQGLTK HMTVKNKYAT SKHAKISTLP QLNSALVQDL AKAVAKV |
| Expression System: | E. coli |
| Protein Length: | 1-433aa |
| Concentrations: | 0.25 mg/ml (determined by Bradford assay) |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | CCNB1 cyclin B1 [ Homo sapiens ] |
| Gene ID NCBI: | 891 |
| Synonyms: | CCNB1; cyclin B1; CCNB; G2/mitotic-specific cyclin-B1; G2/mitotic specific cyclin B1; G2/mitotic-specific cyclin B1; |
| mRNA Refseq: | NM_031966 |
| Protein Refseq: | NP_114172 |
| MIM: | 123836 |
| UniProt ID: | P14635 |
| Chromosome Location: | 5q12 |
| Function: | histone kinase activity; kinase activity; patched binding; protein binding; protein kinase binding; protein kinase binding; |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0060 | Recombinant Human CCND1 293 Cell Lysate | Inquiry |
| EL-0120 | Recombinant Human CCNB1 Cell Lysate | Inquiry |
| EL-0197 | Recombinant Human CCDC101 293 Cell Lysate | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-0515 | Recombinant Human Cyclin D1, GST-tagged | Inquiry |
| PE-0516 | Recombinant Human CCND1, His-tagged | Inquiry |
| Related Gene / Proteins | |||
| CCDC101 | CCL14 | CCNB1 | CCND1 |
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