| Cat.No.: | PE-0856 |
| Product Name: | Recombinant Human RELA, His-tagged |
| Product Overview: | Recombinant Human RELA isproduced in Sf21 cells and Fused to a His-tag. |
| Description: | NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine). |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | ≥95% (SDS-PAGE). |
| Species: | Human |
| Formulation: | Liquid. In 50mM TRIS-HCl, pH 7.5, containing 100mM sodium chloride, 0.2% NP-40, 50-100mM imidazole and 10% glycerol. |
| Tag: | His |
| Activity: | ~1ng is required for high mobility shift assay. ~5ng are required for reconstituted transcription assays. |
| Expression System: | Sf21 Insect Cells |
| Concentrations: | 0.5mg/ml. |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | RELA v-rel reticuloendotheliosis viral oncogene homolog A (avian) [ Homo sapiens ] |
| Gene ID NCBI: | 5970 |
| Synonyms: | RELA; v-rel reticuloendotheliosis viral oncogene homolog A (avian); nuclear factor NF-kappa-B p65 subunit; nuclear factor of kappa light polypeptide gene enhancer in B-cells 3; transcription factor p65; v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)); v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65; p65; NFKB3; MGC131774 |
| mRNA Refseq: | NM_001145138 |
| Protein Refseq: | NP_001138610 |
| MIM: | 164014 |
| UniProt ID: | Q04206 |
| Chromosome Location: | 11q13 |
| Function: | DNA binding; NF-kappaB binding; promoter binding; protein binding; transcription factor activity; RNA polymerase II transcription factor activity, enhancer binding; identical protein binding; protein complex binding; protein binding; transcription activator binding; transcription factor binding; transcription activator activity; transcription repressor binding; transcription repressor activity |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0113 | Recombinant Human RELA 293 Cell Lysate | Inquiry |
| ◆ Research Kits | ||
| EKIT-0275 | HEK 293 RELA Nuclear Translocation Assay Kit | Inquiry |
| ◆ Cell Lines | ||
| CL-0396 | Human RECQL4 Knockout Cell Line 5bp deletion | Inquiry |
| ◆ Proteins & Enzymes | ||
| PE-0856 | Recombinant Human RELA, His-tagged | Inquiry |
| PE-0857 | Recombinant Human RELA | Inquiry |
| Related Gene / Proteins | |||
| RECQL4 | rela | Reptin | REST |
| REXO5 | |||
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