| Cat.No.: | PE-0636 |
| Product Name: | Recombinant Human K (lysine) Acetyltransferase 2B |
| Description: | CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. PCAF belongs to the GCN5/PCAF family of nuclear HATs. The proteins consist of N-terminal and C-terminal domains separated by a deep hydrophobic cleft. The C-terminus contains the HAT domain and a bromodomain, while the Nterminus contains a PCAF-specific domain. Numerous studies indicate that these HATs function as histone-acetylating transcriptional co-activators. Besides histones, PCAF also acetylates non-histone proteins. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. |
| Applications: | Use only for Research. Recombinant PCAF can be used: (1) for protein-protein raction assay. (2)For in vitro transcription assay. (3) For in vitro acetylation assay. (4) For cell growth assay. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Purity: | >95% by SDS-PAGE. |
| Species: | Human |
| Formulation: | Liquid. Supplied in 20mM Tris-HCl pH8.0, 20% glycerol, 100mM KCl, 0.2mM EDTA and 1mM DTT. |
| Activity: | 1-5ng are sufficient for a gel mobility shift assay in a 20μl reaction, 50-100ng are sufficient for reconstituted transcription assay and 100-200ng are sufficient for a protein-protein interaction assay or an acetylation assay. |
| Expression System: | Sf9 Insect Cells |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | KAT2B K(lysine) acetyltransferase 2B [ Homo sapiens ] |
| Gene ID NCBI: | 8850 |
| Synonyms: | KAT2B; K(lysine) acetyltransferase 2B; P; CAF; PCAF; P/CAF; CREBBP-associated factor; p300/CBP-associated factor; Histone acetyltransferase KAT2B; EC 2.3.1.48; Lysine acetyltransferase 2B; Histone acetylase PCAF; P300/CBP-associated factor; P/CAF; GCN5; GCN5L |
| mRNA Refseq: | NM_003884 |
| Protein Refseq: | NP_003875 |
| MIM: | 602303 |
| UniProt ID: | Q92831 |
| Chromosome Location: | 3p24 |
| Function: | histone acetyltransferase activity; histone deacetylase binding; transcription coactivator activity; transferase activity |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0073 | Anacardic Acid | Inquiry |
| ◆ Antibodies | ||
| EAb-0073 | KAT8 Polyclonal Antibody | Inquiry |
| ◆ Cell Lines | ||
| CL-0083 | Human KAT2A Knockout Cell Line 1bp insertion | Inquiry |
| CL-0085 | Human KAT2B Knockout Cell Line 31bp deletion | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0159 | Recombinant Human MYST2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| Kaiso | KANSL2 | KAP1 | KAT13A |
| KAT13D | KAT2A | KAT2B | KAT4 |
| KAT5 | KAT6A | KAT6B | KAT7 |
| KAT8 | KAT9 | ||
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
