| Cat.No.: | PE-0162 |
| Product Name: | Recombinant Human SIRT3, His-tagged |
| Product Overview: | Recombinant Human SIRT3(Ser101-Lys399), fused with an N-terminal Met and 6-His tag, was expressed in E. coli. |
| Description: | This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Two alternatively spliced transcript variants that encode different proteins have been described for this gene. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | Predicted Molecular Mass: 34 kDaSDS-PAGE: 30-33 kDa, reducing conditions |
| Purity: | >95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie Blue stain at 5 µg per lane. |
| Species: | Human |
| Formulation: | Supplied as a 0.2 µM filtered solution in Tris, NaCl, Brij-35 and Glycerol. |
| Tag: | His |
| Endotoxin: | < 1.0 EU per 1 μg of the protein by the LAL method. |
| Activity: | Measured by its ability to remove the acetyl group from a fluorogenic peptide substrate Ac-RGK(Ac)-AMC in a coupled assay. |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | SIRT3 sirtuin 3 [ Homo sapiens (human) ] |
| Gene ID NCBI: | 23410 |
| Synonyms: | SIRT3; sirtuin (silent mating type information regulation 2 homolog) 3 (S. cerevisiae); SIR2L3; NAD-dependent deacetylase sirtuin-3, mitochondrial; EC 3.5.1.-; SIR2-like protein 3; hSIRT3; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase 2; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; sirtuin (silent mating type information regulation 2, S.cerevisiae, homolog) 3; sirtuin 3; sirtuin type 3 |
| mRNA Refseq: | NM_001017524 |
| Protein Refseq: | NP_001017524 |
| MIM: | 604481 |
| Chromosome Location: | 11p15.5 |
| Function: | NOT NAD+ ADP-ribosyltransferase activity; NAD-dependent histone deacetylase activity (H3-K14 specific); protein binding |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
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