| Cat.No.: | PE-0130 |
| Product Name: | Recombinant Human SIRT1 (Active), His-tagged |
| Product Overview: | Recombinant human SIRT1 (193-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. |
| Description: | SIRT1 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor. It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein. Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation. |
| Applications: | SIRT Assay |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | ~84 kDa |
| Purity: | >70% |
| Species: | Human |
| Formulation: | Recombinant protein stored in 50mM sodium phosphate, pH 7.0, 300mM NaCl, 150mM imidazole, 0.1mM PMSF, 0.25mM DTT, 25% glycerol. |
| Tag: | His |
| Activity: | 2100 RLU/min/ng |
| Expression System: | Sf9 Insect Cells |
| Protein Length: | 193 aa-end |
| Concentrations: | 0.1μg/μl |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | SIRT1 sirtuin 1 [ Homo sapiens ] |
| Gene ID NCBI: | 23411 |
| Synonyms: | SIRT1; sirtuin 1; sirtuin (silent mating type information regulation 2 homolog) 1 (S. cerevisiae) , sirtuin (silent mating type information regulation 2, S. cerevisiae, homolog) 1; NAD-dependent deacetylase sirtuin-1; SIR2L1; hSIR2; hSIRT1; SIR2alpha; sir2-like 1; sirtuin type 1; SIR2-like protein 1; |
| mRNA Refseq: | NM_001142498 |
| Protein Refseq: | NP_001135970 |
| MIM: | 604479 |
| UniProt ID: | Q96EB6 |
| Chromosome Location: | 10q21 |
| Function: | HLH domain binding; NOT NAD+ ADP-ribosyltransferase activity; NAD+ binding; NAD-dependent histone deacetylase activity; NAD-dependent histone deacetylase activity (H3-K9 specific); NAD-dependent protein deacetylase activity; NAD-dependent protein deacetylase activity; bHLH transcription factor binding; deacetylase activity; enzyme binding; histone binding; histone deacetylase activity; identical protein binding; metal ion binding; mitogen-activated protein kinase binding; p53 binding; protein C-terminus binding; protein binding; protein deacetylase activity; transcription corepressor activity; transcription corepressor activity; zinc ion binding; |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
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