| Cat.No.: | PE-0069 |
| Product Name: | Recombinant Human HDAC5 Protein, His-tagged |
| Product Overview: | Recombinant Human HDAC5 fused with His tag was expressed in Insect cells. |
| Description: | HDAC5 is a member of the histone deacetylase family. It is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC5 possesses histone deacetylase activity and represses transcription when tethered to a promoter. It coimmunoprecipitates only with HDAC3 family member and might form multicomplex proteins. It also interacts with myocyte enhancer factor-2 (MEF2) proteins, resulting in repression of MEF2-dependent genes. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 52.2 kDa, observed by reducing SDS-PAGE. |
| Purity: | > 90% by SDS-PAGE analysis. |
| Species: | Human |
| Formulation: | The HDAC5 solution contains 20 mM Tris-HCl buffer (pH 8.0), 300 mM NaCl and 20% Glycerol. Frozen solution. |
| Tag: | His |
| Amino Acid Sequence: | MGRTQSSPAA PGGMKSPPDQ PVKHLFTTGV VYDTFMLKHQ CMCGNTHVHP EHAGRIQSIW SRLQETGLLS KCERIRGRKA TLDEIQTVHS EYHTLLYGTS PLNRQKLDSK KLLGPISQKM YAVLPCGGIG VDSDTVWNEM HSSSAVRMAV GCLLELAFKV AAGELKNGFA IIRPPGHHAE ESTAMGFCFF NSVAITAKLL QQKLNVGKVL IVDWDIHHGN GTQQAFYNDP SVLYISLHRY DNGNFFPGSG APEEVGGGPG VGYNVNVAWT GGVDPPIGDV EYLTAFRTVV MPIAHEFSPD VVLVSAGFDA VEGHLSPLGG YSVTARCFGH LTRQLMTLAG GRVVLALEGG HDLTAICDAS EACVSALLSV ELQPLDEAVL QQKPNINAVA TLEKVIEIQS KHWSCVQKFA AGLGRSLREA QAGETEEAET VSAMALLSVG AEQAQAAAAR EHSPRPAEEP MEQEPALHHH HHHHHHH |
| Endotoxin: | < 1 EU/μg, determined by LAL method. |
| Activity: | > 10000 pmol/min/μg. Assay condition: 50 mM Tris-HCl, pH8.0, 136 mM NaCl, 2.8 mM KCl, 1 mM MgCl2, 0.5 mg/ml BSA, 0.005% Tween-20, 20 M AMC-RHKKAc and HDAC5. HDAC reaction was done for 30 min at 37 centigrade followed by treatment with equivoluminal HDAC developer buffer (40 μM Trypsin, 40 μM CaCl2 and 20 μM Trichostatin A for ~60 min at RT. |
| Expression System: | Insect Cells |
| Protein Length: | 476 amino acids |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Gene Name: | HDAC5 histone deacetylase 5 [ Homo sapiens ] |
| Gene ID NCBI: | 10014 |
| Official Symbol: | HDAC5 |
| Synonyms: | HDAC5; histone deacetylase 5; FLJ90614; KIAA0600; NY CO 9; antigen NY-CO-9; HD5; NY-CO-9; |
| mRNA Refseq: | NM_005474 |
| Protein Refseq: | NP_005465 |
| MIM: | 605315 |
| UniProt ID: | Q9UQL6 |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0006 | Recombinant Human HDAC1 293 Cell Lysate | Inquiry |
| EL-0007 | Recombinant Human HDAC2 293 Cell Lysate | Inquiry |
| EL-0008 | Recombinant Human HDAC3 Cell Lysate | Inquiry |
| EL-0009 | Recombinant Human HDAC4 Cell Lysate | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0007 | Tubacin | Inquiry |
| Related Gene / Proteins | |||
| HDAC | HDAC1 | HDAC10 | HDAC11 |
| HDAC2 | HDAC3 | hdac4 | hdac5 |
| HDAC6 | hdac7 | HDAC8 | hdac9 |
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