Cat.No.: | EAb-1897 |
Product Name: | NFκB p65 phospho Ser536 Polyclonal Antibody |
Antibody Type: | Polyclonal |
Immunogen: | This NFkB p65 phospho Ser536 antibody was raised against a peptide containing phospho-serine 536 of human NFkB p65. |
Host: | Rabbit |
Isotype: | Serum |
Molecular Weight: | 65 kDa |
Purification: | None |
Appearance: | Liquid |
Formulation: | Rabbit serum containing 30% glycerol and 0.035% sodium azide. |
Applications: | WB: 1:500 - 1:1,000 dilution^Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
Species Reactivity: | Human |
Storage: | -20°C |
Warning: | For Research Use Only! Not For Use in Humans. |
Scientific Background: | NFκB p65 (Nuclear factor NF-kappa-B p65 subunit also known as NFκB p65 or RelA) is a member of the Rel family of transcription factors that are involved in processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. The Rel family includes c-Rel, RelA (NFκB p65, NFκB3) and RelB (I-Rel). Rel proteins form dimers with the NFκB p50 (proteolytically processed from p105/NFκB1) and p52 (proteolytically processed from p100/NFκB2) members of the NFκB family that bind DNA and activate transcription. The NFκB p65/p50 heterodimer is the most abundant form of NFκB.NFκB signaling is negatively regulated by the sequestration of the NFκB complex in the cytoplasm by its association with the IκB family of inhibitory proteins. The IκB Kinase (IKK) complex is the key enzyme involved in the activation and translocation of NFκB. The IKK complex is composed of two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (IKKγ). Upon its activation, the IKK complex phosphorylates IκB proteins (IκBα, IκBα phospho Ser32, 36), which marks them for ubiquitination and degradation. This enables the NFκB complex to translocate to the nucleus where it regulates transcription by binding to DNA. Phosphorylation at serine 536 stimulates acetylation at lysine 310 and interaction with the acetyltransferase CBP. The phosphorylated and acetylated forms show enhanced transcriptional activity. |
Product Types | ||
◆ Research Kits | ||
EKIT-0429 | NFkB p65 Transcription Factor Assay Kit | Inquiry |
◆ Antibodies | ||
EAb-1897 | NFκB p65 phospho Ser536 Polyclonal Antibody | Inquiry |
EAb-1898 | NFκB p65 phospho Ser529 Polyclonal Antibody | Inquiry |
EAb-1899 | NFκB p65 Polyclonal Antibody | Inquiry |
EAb-1900 | NFκB p65 Polyclonal Antibody | Inquiry |
Related Gene / Proteins | |||
p65 |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools