| Cat.No.: | EAb-1839 |
| Product Name: | RNA Pol II CTD phospho Tyr1 Monoclonal Antibody |
| Antibody Type: | Monoclonal |
| Immunogen: | This antibody was raised against a synthetic peptide containing the RNA Pol II heptad repeat consensus sequence phosphorylated at tyrosine 1. |
| Host: | Rat |
| Isotype: | IgG1 |
| Molecular Weight: | 210 kDa |
| Purification: | Protein G Chromatography |
| Appearance: | Liquid |
| Formulation: | Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. |
| Applications: | WB: 1 – 2 µg/ml^Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Human, Wide Range Predicted |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Scientific Background: | RNA pol II (RNA polymerase II) is responsible for synthesizing messenger RNA in eukaryotes. RNA pol II contains a carboxy terminal domain composed of heptapeptide repeats that are essential for polymerase activity. These repeats contain serine and threonine residues that are phosphorylated in actively transcribing RNA polymerase. In addition, RNA pol II, in combination with several other polymerase subunits, form the DNA binding domain of the polymerase, a groove in which the DNA template is transcribed into RNA. During the transcription cycle, the CTD of the large subunit of RNA pol II is reversibly phosphorylated. RNA pol II containing unphosphorylated CTD is recruited to the promoter, whereas the hyperphosphorylated CTD form is involved in active transcription. Phosphorylation occurs at sites within the heptapeptide repeat, at serine 2, serine 5, serine 7 and tyrosine 1. RNA pol II Tyrosine 1 phosphorylation is found during elongation and appears to activate transcription by suppressing RNA pol II termination. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0123 | Human RNF167 Knockout Cell Line | Inquiry |
| CL-0124 | Human RNF2 Knockout Cell Line 553bp insertion | Inquiry |
| CL-0125 | Human RNF25 Knockout Cell Line | Inquiry |
| CL-0126 | Human RNF26 Knockout Cell Line | Inquiry |
| CL-0127 | Human RNF24 Knockout Cell Line | Inquiry |
| Related Gene / Proteins | |||
| RNA Helicase A | RNA pol II | RNF103 | RNF11 |
| RNF128 | RNF133 | RNF138 | RNF141 |
| RNF167 | RNF168 | RNF181 | RNF182 |
| RNF183 | RNF2 | RNF20 | RNF207 |
| RNF208 | RNF212 | RNF213 | RNF214 More > |
| RNF215 | RNF217 | RNF219 | RNF220 |
| RNF222 | RNF223 | RNF224 | RNF24 |
| RNF25 | RNF26 | RNF31 | RNF40 |
| RNF7 | RNF8 | RNP | RNPC3 |
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