| Cat.No.: | EAb-1536 |
| Product Name: | Ubiquitin Polyclonal Antibody |
| Antibody Type: | Polyclonal |
| Conjugate: | Unconjugated |
| Host: | Rabbit |
| Isotype: | IgG |
| Purification: | Affinity Purified |
| Appearance: | Liquid |
| Formulation: | 1*TBS (pH7.4), 0.5%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| Applications: | ICC, IHC, WB |
| Recommended Dilutions/Conditions: |
IHC, WB: (1:1000-1:2000), ICC: (1:200) Recommended dilutions/conditions may not be available for all applications. Specific conditions for reactivity should be optimized by the end user. |
| Species Reactivity: | Bovine, Chicken, Dog, Drosophila, Fish, Guinea pig, Hamster, Human, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus, Yeast |
| Storage: | -20°C |
| Warning: | For Research Use Only! Not For Use in Humans. |
| Scientific Background: | Ubiquitin is a small regulatory protein that has been found in almost all tissues of eukaryotic organisms. The ubiquitin protein consists of 76 amino acids and has a molecular mass of approximately 8.5 kDa. This protein’s main features include a C-terminal tail and the 7 Lys residues. Ubiquitin is highly conserved among all eukaryotic species. Remarkably, human ubiquitin shares nearly the same sequence identity (96%) as yeast ubiquitin. The primary function of this protein is to get rid of foreign, abnormal, and improperly folded proteins by targeting them for degradation via the 26S proteasome. The addition of ubiquitin to a substrate protein is called ubiquitination. Ubiquitination is an important cellular process affected by a multi-enzyme cascade. These enzymes include ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin-protein ligases (E3s). Ubiquitination affects proteins in various ways: it can signal for their degradation via the proteasome, change their cellular location, affect their activity, and promote or prevent protein interactions. Ubiquitination is carried out in three main steps: activation, conjugation, and ligation, performed by E1s, E2s, and E3s respectively. The consequence of this cascade binds ubiquitin to lysine residues on the protein substrate using an isopeptide bond, or it may bind the amino group of the protein's N-terminus using a peptide bond. The protein modifications can be either a single ubiquitin protein (monoubiquitination) or a chain of ubiquitin (polyubiquitination). In addition, ubiquitin has been found to participate in regulating signal transduction cascades through the elimination inhibitor proteins. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0026 | Human UBAP1 Knockout Cell Line | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0162 | Synthetic Human Ubiquitin protein (Biotin) | Inquiry |
| SP-0163 | Human Ubiquitin peptide | Inquiry |
| ◆ Antibodies | ||
| EAb-0174 | UBE2G1 Polyclonal Antibody | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0315 | NSC 697923 | Inquiry |
| Related Gene / Proteins | |||
| UB2D1 | UB2D2 | UB2D3 | UB2R1 |
| UBA1 | UBA5 | UBA7 | UBAP1 |
| UBB | UBC | UBC12 | Ubc13 |
| Ubc3B | UbcH1 | UbcH2 | UbcH3 |
| UbcH5a | UbcH5b | UbcH5c | UbcH8 More > |
| UBD | UBE1L | UBE2B | UBE2C |
| UBE2D1 | UBE2D3 | UBE2DNL | UBE2E1 |
| UBE2E2 | UBE2E3 | UBE2G1 | UBE2G2 |
| UBE2H | UBE2I | UBE2K | UBE2L3 |
| UBE2L6 | UBE2M | UBE2N | UBE2O |
| UBE2Q2 | UBE2R1 | UBE2R2 | UBE2T |
| UBE2V1 | UBE2V2 | UBE2W | UBE3A |
| Ube4a | Ubiquitin | UBL7 | Ubn1 |
| UBP5 | UBR2 | UBTD1 | UBTD2 |
| UBXN10 | UBXN2B | UBXN6 | UBXN8 |
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